College of Forestry, Northwest A&F University, Yangling 712100, Shaanxi, China.
Jiangsu Co-Innovation Center of Efficient Processing and Utilization of Forest Resources, College of Chemical Engineering, Nanjing Forestry University, Nanjing 210037, China; Key Laboratory of Forestry Genetics & Biotechnology (Nanjing Forestry University), Ministry of Education, Nanjing 210037, China.
Bioresour Technol. 2021 Mar;323:124593. doi: 10.1016/j.biortech.2020.124593. Epub 2020 Dec 30.
Recently, efficient production of xylo-oligosaccharides (XOS) from poplar by acetic acid (AA) pretreatment was developed; but the effect of residual lignin on subsequent cellulase hydrolysis was unclear. Herein, XOS was produced from poplar by AA pretreatment and the effect of AA pretreatment on lignin inhibition to cellulase hydrolysis was investigated. The results indicated that a high XOS yield of 55.8% was obtained, and the inhibition degree of lignin in poplar increased from 1.0% to 6.8% after AA pretreatment. Lignin was acetylated and its molecular weight decreased from 12,211 to 2871 g/mol after AA pretreatment. The increase of S/G ratio, phenolic hydroxyl, and condensed units of lignin after AA pretreatment might be reasons for this intensified inhibition. The results advanced our understanding of the structural and inhibitory properties of lignin after production of XOS from poplar with AA pretreatment, and provided references for efficient cellulase hydrolysis of poplar after AA pretreatment.
最近，通过乙酸（AA）预处理从杨树中高效生产木二糖（XOS）得到了发展；但残余木质素对后续纤维素酶水解的影响尚不清楚。本文通过 AA 预处理从杨树中生产 XOS，并研究了 AA 预处理对木质素抑制纤维素酶水解的影响。结果表明，获得了 55.8%的高 XOS 产率，并且 AA 预处理后，杨树中木质素的抑制程度从 1.0%增加到 6.8%。AA 预处理后，木质素被乙酰化，其分子量从 12211 降低到 2871g/mol。木质素的 S/G 比、酚羟基和缩合单元增加可能是这种抑制增强的原因。这些结果增进了我们对 AA 预处理生产 XOS 后木质素的结构和抑制特性的理解，为 AA 预处理后杨树的高效纤维素酶水解提供了参考。
