Department of Civil and Environmental Engineering, Shantou University, No. 243 Daxue Road, Shantou, Guangdong Province, 515063, China.
School of Environmental Science and Engineering, Tiangong University, No. 399 Binshui West Road, Xiqing District, Tianjin, 300387, China.
Chemosphere. 2021 May;270:129428. doi: 10.1016/j.chemosphere.2020.129428. Epub 2020 Dec 28.
We used a pot experiment to investigate the effectiveness of 0.5, 1.0, and 2.0% biochar (BC) or iron-manganese oxide modified biochar (FMBC) additions on the biomass, enzyme activity, and grain quality of wheat plants grown in dibutyl phthalate (DBP) and di-(2-ethylhcxyl) phthalate (DEHP) polluted fluvo-aquic soils, as well as the bioavailability of DBP and DEHP. BC and FMBC applications significantly reduced DBP and DEHP accumulation in grains, which enhanced the content of starch and protein-related enzyme, thereby improving yield, and starch and protein content in wheat grains and increasing the content of minerals including Fe, Mn, K and Ca. Molecular docking assays showed that DBP and DEHP could bind to starch synthase (GBSS) through hydrogen bonds and intermolecular forces, which may have hindered the entry of substrates or occupied the binding sites of the reactants, thus inhibiting the activity of GBSS. In addition, FMBC treatment had a better inhibitory effect on the phytotoxicity of DBP and DEHP on wheat grain than BC treatment. This result might be attributed to the fact that FMBC has more functional groups and porous structure, and larger specific surface area. In summary, these findings contribute to our understanding of the mechanism of phthalate phytotoxicity, which may help us prevent/reduce it in the future.
我们采用盆栽试验研究了 0.5%、1.0%和 2.0%生物炭（BC）或铁锰氧化物改性生物炭（FMBC）添加对受邻苯二甲酸二丁酯（DBP）和邻苯二甲酸二（2-乙基己基）酯（DEHP）污染的潮土和潮土中种植的小麦植株的生物量、酶活性和籽粒品质，以及 DBP 和 DEHP 的生物有效性。BC 和 FMBC 的应用显著降低了 DBP 和 DEHP 在谷物中的积累，提高了淀粉和与蛋白质相关的酶的含量，从而提高了小麦籽粒的产量和淀粉及蛋白质含量，并增加了 Fe、Mn、K 和 Ca 等矿物质的含量。分子对接实验表明，DBP 和 DEHP 可以通过氢键和分子间力与淀粉合酶（GBSS）结合，这可能阻碍了底物的进入或占据了反应物的结合位点，从而抑制了 GBSS 的活性。此外，FMBC 处理对 DBP 和 DEHP 对小麦籽粒的植物毒性的抑制作用优于 BC 处理。这一结果可能归因于 FMBC 具有更多的官能团和多孔结构，以及更大的比表面积。综上所述，这些发现有助于我们理解邻苯二甲酸酯植物毒性的机制，这可能有助于我们在未来预防/减少这种毒性。
