Gomez Johana S, Parada Romina B, Vallejo Marisol, Marguet Emilio R, Bellomio Augusto, Perotti Nora, de Carvalho Kátia G
Planta Piloto de Procesos Industriales Microbiológicos (PROIMI), CONICET, Av. Belgrano y Pje. Caseros, San Miguel de Tucumán, 4000, Tucumán, Argentina.
Laboratorio de Biotecnología Bacteriana, Fac. de Cs. Naturales y Cs. de la Salud - UNPSJB, Sede Trelew, Chubut, Argentina.
Arch Microbiol. 2021 May;203(4):1427-1437. doi: 10.1007/s00203-020-02118-0. Epub 2021 Jan 2.
Lactic acid bacteria can be considered as natural biopreservative and good biotechnological alternative to food safety. In this study, the antilisterial compounds produced by Enterococcus isolates from the Patagonian environment and their effectiveness for the control of Listeria monocytogenes in a food model were studied. Enterococcus isolates whose cell-free supernatant presented activity against Listeria monocytogenes were identified and evaluated for their virulence factors. The activity of the antimicrobial compounds produced by Enterococcus sp. against Listeria monocytogenes Scott A in meat gravy and ground beef during refrigerated storage was tested. The results indicated that ten Enterococcus isolates presented activity against Listeria monocytogenes and none of the selected strains presented virulence factors. L. monocytogenes in the food models containing the antilisterial compounds produced by Enterococcus sp. has decreased over the days, indicating that these compounds and cultures are an alternative to control the growth of L. monocytogenes in foods.
乳酸菌可被视为天然生物防腐剂，是食品安全方面良好的生物技术替代品。在本研究中，对从巴塔哥尼亚环境中分离出的肠球菌产生的抗李斯特菌化合物及其在食品模型中控制单核细胞增生李斯特菌的有效性进行了研究。鉴定了无细胞上清液对单核细胞增生李斯特菌具有活性的肠球菌分离株，并对其毒力因子进行了评估。测试了肠球菌产生的抗菌化合物在冷藏储存期间对肉汁和碎牛肉中单核细胞增生李斯特菌斯科特A的活性。结果表明，10株肠球菌分离株对单核细胞增生李斯特菌具有活性，所选菌株均未呈现毒力因子。含有肠球菌产生的抗李斯特菌化合物 的食品模型中的单核细胞增生李斯特菌数量在数天内有所减少，表明这些化合物和培养物是控制食品中单核细胞增生李斯特菌生长的一种替代方法。
