University of the Chinese Academy of Sciences, Beijing, 100049, China.
Beijing National Laboratory for Molecular Sciences, Key Laboratory of Analytical Chemistry for Living Biosystems, Institute of Chemistry, Chinese Academy of Sciences, Beijing, 100190, China.
Anal Bioanal Chem. 2021 Feb;413(4):1099-1106. doi: 10.1007/s00216-020-03072-y. Epub 2021 Jan 3.
We develop a capillary-paper spray (CPS) ion source which allows for sample separation in the capillary and enables rapid and sensitive paper spray (PS) mass spectrometry (MS) analysis of biofluids. The CPS employs a glass capillary to load liquid analytes, vertically standing at the rear of the PS. To further reduce the matrix effect, a nitrocellulose filter membrane is placed between the glass tube and chromatography paper to absorb proteins and other macromolecules, which is beneficial for the detection of the small molecules. Compared with the normal PS method, the CPS method markedly improves spray stability and prolongs analysis duration, and also generates significantly better signal intensities during the analysis of drugs, thus indicating its potential for clinical use. As a proof of concept, quantitative analysis of drugs (metformin hydrochloride and berberine hydrochloride) in serum is performed.
我们开发了一种毛细管-纸喷雾（CPS）离子源，它允许在毛细管中进行样品分离，并实现了生物流体的快速和灵敏的纸喷雾（PS）质谱（MS）分析。CPS 采用玻璃毛细管加载液体分析物，垂直位于 PS 的后部。为了进一步降低基质效应，在玻璃管和层析纸之间放置硝化纤维素滤膜以吸收蛋白质和其他大分子，这有利于小分子的检测。与正常 PS 方法相比，CPS 方法显著提高了喷雾稳定性并延长了分析时间，并且在药物分析中产生了明显更好的信号强度，这表明其具有临床应用的潜力。作为概念验证，对血清中的药物（盐酸二甲双胍和盐酸小檗碱）进行定量分析。
