Department of Livestock Farm Complex, College of Veterinary Science, Rampura Phul, GADVASU, Ludhiana, India.
Division of Avian Genetics and Breeding, Central Avian Research Institute, Indian Council of Agricultural Research, Bareilly (Uttar Pradesh), India.
Trop Anim Health Prod. 2021 Jan 3;53(1):59. doi: 10.1007/s11250-020-02519-9.
Feed constitutes about 70% of the total expenditure of poultry production. Maximizing the feed efficiency in juvenile period is essential to achieve low production cost. The efficiency of feed utilization was measured by RFI (residual feed intake) by calculating the difference between an individual animal's observed and its expected feed intake. The expression of genes influencing low and high RFI is required to know the basic molecular mechanism influencing feed efficiency. The present study aimed to estimate the RFI (0-5 week) in a population of indigenously developed colored broiler sire line chicken. The duodenum sample of high and low-RFI broiler chicken was used for microarray analysis. Duodenum exhibited 1030 differentially expressed genes after analysis. Out of total DEGs, 461 genes were downregulated and 569 were upregulated. The fold change of differentiallly expressed genes varies from - 162.6 to 1549.28. A subset of genes was validated by qRT-PCR and results were correlated well with microarray data. In functional annotation study of DEGs, 89 biological processes, 30 cellular components, and 29 molecular functions were identified. Study of the important differentially expressed genes and the related molecular pathways in the population may hold the potential for future breeding strategies for augmenting feed efficiency.
饲料约占家禽生产总支出的 70%。在幼年期最大限度地提高饲料效率对于降低生产成本至关重要。饲料利用效率通过 RFI（剩余饲料摄入量）来衡量，方法是计算个体动物观察到的饲料摄入量与其预期饲料摄入量之间的差异。为了了解影响饲料效率的基本分子机制，需要了解影响低 RFI 和高 RFI 的基因的表达。本研究旨在估算本土彩色肉鸡父系鸡群的 RFI（0-5 周）。使用高 RFI 和低 RFI 肉鸡的十二指肠样本进行微阵列分析。分析后，十二指肠显示出 1030 个差异表达基因。在总差异表达基因中，有 461 个下调，569 个上调。差异表达基因的倍数变化范围从-162.6 到 1549.28。通过 qRT-PCR 验证了一部分基因，结果与微阵列数据相关性良好。在 DEGs 的功能注释研究中，确定了 89 个生物学过程、30 个细胞成分和 29 个分子功能。对该群体中重要的差异表达基因及其相关分子途径的研究可能为未来提高饲料效率的育种策略提供潜力。
