香芹酚通过半胱天冬酶依赖性细胞凋亡和细胞周期进程阻断发挥宫颈癌化学预防作用。
Carvacrol Exhibits Chemopreventive Potential against Cervical Cancer Cells via Caspase-Dependent Apoptosis and Abrogation of Cell Cycle Progression.
机构信息
Department of Biosciences, Integral University, Lucknow, 226026, India.
出版信息
Anticancer Agents Med Chem. 2021 Oct 28;21(16):2224-2235. doi: 10.2174/1871520621999201230201258.
BACKGROUND
The carcinogenesis of the uterine cervix is predominantly initiated with the consistent infection of the Human Papilloma Virus (HPV). Owing to the side effects of standard chemotherapeutics in the treatment of recurrent and metastatic cervical cancer, there is a need for a better and effective treatment modality. In this lieu of concern, natural compounds have proven their worthwhile potential against the treatment of various carcinomas. Carvacrol is a phenolic monoterpenoid and several reports have suggested its different biological properties including antioxidant, anti-inflammatory and anticancer activity.
OBJECTIVE
The objective of our present study was to investigate the effect of carvacrol on HPV18+ HeLa cervical cancer cells.
METHODS
HeLa cervical cancer cells were cultured and subsequently treated with various doses of carvacrol. Cell viability was assessed via MTT assay. DAPI and Hoechst3342 staining were used to qualitatively analyzed the induced apoptosis. Reactive Oxygen Species (ROS) was estimated by DCFDA staining protocol and quantitatively estimated by flow cytometry. The cell cycle distribution and apoptosis (FITC-Annexin V assay) were analyzed by flow cytometry.
RESULTS
The results of the present study have established that carvacrol strongly suppresses the proliferation of cervical cancer cells via caspase-dependent apoptosis and abrogation of cell cycle progression. Furthermore, our preliminary study also demonstrated that carvacrol exhibits a synergistic effect with chemotherapeutic drugs (5-FU and carboplatin). These initial findings implicated that natural compounds could reduce the toxic effects of chemotherapeutic drugs.
CONCLUSION
Therefore, this investigation affirms the anti-cancer potential of carvacrol against cervical cancer cells, which could be an appendage in the prevention and treatment of cervical cancer.
背景
子宫颈的癌变主要是由人乳头瘤病毒（HPV）的持续感染引发的。由于标准化学疗法在治疗复发性和转移性宫颈癌方面的副作用，因此需要更好、更有效的治疗方法。鉴于此，天然化合物已被证明对治疗各种癌具有有价值的潜力。香芹酚是一种酚类单萜，有几项报告表明其具有不同的生物学特性，包括抗氧化、抗炎和抗癌活性。
目的
本研究的目的是研究香芹酚对 HPV18+HeLa 宫颈癌细胞的影响。
方法
培养 HeLa 宫颈癌细胞，然后用不同剂量的香芹酚处理。通过 MTT 测定法评估细胞活力。使用 DAPI 和 Hoechst3342 染色定性分析诱导的细胞凋亡。通过 DCFDA 染色方案估计活性氧（ROS），并通过流式细胞术进行定量估计。通过流式细胞术分析细胞周期分布和细胞凋亡（FITC-Annexin V 检测）。
结果
本研究的结果表明，香芹酚通过 caspase 依赖性细胞凋亡和细胞周期进程的阻断强烈抑制宫颈癌细胞的增殖。此外，我们的初步研究还表明，香芹酚与化疗药物（5-FU 和卡铂）表现出协同作用。这些初步发现表明，天然化合物可以降低化疗药物的毒性作用。
结论
因此，这项研究证实了香芹酚对宫颈癌细胞的抗癌潜力，这可能是预防和治疗宫颈癌的一种辅助手段。
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