一种即时聚合酶链反应检测方法的验证，用于检测疑似马传染性鼻疽病马的鼻拭子中亚种。
Validation of a point-of-care polymerase chain reaction assay for detection of subspecies in rostral nasal swabs from horses with suspected strangles.
机构信息
William R. Pritchard Veterinary Medical Teaching Hospital, School of Veterinary Medicine, University of California, 1 Garrod Drive, Davis, California, USA (Willis); Department of Medicine and Epidemiology, School of Veterinary Medicine, University of California, One Shields Avenue, Davis, California, USA (Barnum, Pusterla).
出版信息
Can Vet J. 2021 Jan;62(1):51-54.
This study aimed to validate a point-of-care polymerase chain reaction (PCR) assay for detection of subsp. in rostral nasal swabs from horses with suspected acute strangles and to compare the results against the molecular gold standard of quantitative polymerase chain reaction (qPCR). Two hundred thirty-two individual swabs of rostral nasal passages were characterized by qPCR as positive, subsp. positive, or and negative. The specificity and sensitivity of the point-of-care PCR assay were 89% and 84%, respectively. The limits of detection of the qPCR assay and the point-of-care PCR analyzer were 3 and 277 target genes of , respectively. Overall agreement and short turnaround time make the point-of-care PCR assay a potential molecular diagnostic platform that will enhance the capability of equine veterinarians to timely support a diagnosis of strangles and institute proper biosecurity protocols.
本研究旨在验证一种即时聚合酶链反应（PCR）检测方法，用于检测疑似急性马传染性窦炎的马的鼻拭子中的 亚种，并将结果与定量聚合酶链反应（qPCR）的分子金标准进行比较。232 份鼻拭子样本通过 qPCR 鉴定为 阳性、 亚种阳性、 或 阴性。即时 PCR 检测方法的特异性和敏感性分别为 89%和 84%。qPCR 检测方法和即时 PCR 分析仪的检测限分别为 3 和 277 个 目标基因。总体一致性和较短的周转时间使即时 PCR 检测方法成为一种潜在的分子诊断平台，将增强马兽医及时支持窦炎诊断和实施适当生物安全协议的能力。
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