Institute of Medicine, Chung Shan Medical University, Taichung, 40201, Taiwan, ROC.
Department of Medical Laboratory and Biotechnology, Chung Shan Medical University, Taichung, 40201, Taiwan, ROC.
Int J Med Sci. 2021 Jan 1;18(1):157-168. doi: 10.7150/ijms.49423. eCollection 2021.
Bertol. is an herbal plant that belongs to the genus , which is commonly used in traditional medicine to refresh the mind and for diuretic use. However, few studies have reported the function of Bertol. Hence, this study aimed to investigate the anti-tumor and synergistic potential of Bertol. extract (JIB extract) for melanoma cells. Our results indicated the anti-melanoma activity of JIB extract. JIB extract induced cell cycle arrest at the G/G phase and decreased cyclin and cdk protein expressions. In addition, AKT/mTOR signaling and MAPK signaling were inhibited by JIB extract to suppress melanoma cell growth and proliferation. Additionally, JIB extract induced B16/F10 cell apoptosis via the caspase cascade. According to the JIB extract's anti-melanoma capacity, to assess the synergistic effects of cisplatin and JIB extract. The results demonstrated that JIB extract combined with cisplatin enhanced the inhibition of cell growth, proliferation, and survival through the obstruction of cell cycle progression and AKT/mTOR and MAPK signaling as well as the induction of cell apoptosis. Collectively, our results indicate that JIB extract showed anti-tumor effects and synergized with cisplatin against B16/F10 cells, indicating the possibility of JIB extract to be developed as adjuvant therapy for melanoma.
博尔托。是一种属于 的草药植物，在传统医学中常用于提神和利尿。然而，很少有研究报道 博尔托。的功能。因此，本研究旨在探讨 博尔托。提取物（JIB 提取物）对黑素瘤细胞的抗肿瘤和协同作用。我们的结果表明 JIB 提取物具有抗黑色素瘤活性。JIB 提取物诱导细胞周期停滞在 G/G 期，并降低细胞周期蛋白和 CDK 蛋白的表达。此外，JIB 提取物通过抑制 AKT/mTOR 信号和 MAPK 信号通路来抑制黑素瘤细胞的生长和增殖。此外，JIB 提取物通过半胱天冬酶级联诱导 B16/F10 细胞凋亡。根据 JIB 提取物的抗黑色素瘤能力，评估顺铂和 JIB 提取物的协同作用。结果表明，JIB 提取物与顺铂联合使用通过阻止细胞周期进程和 AKT/mTOR 和 MAPK 信号通路以及诱导细胞凋亡来增强对细胞生长、增殖和存活的抑制作用。总之，我们的结果表明，JIB 提取物表现出抗肿瘤作用，并与顺铂协同作用于 B16/F10 细胞，表明 JIB 提取物有可能开发为黑色素瘤的辅助治疗药物。
