Department of Clinical Laboratory Center, The Second Hospital of Lanzhou University, Lanzhou 730000, P.R. China.
The First School of Clinical Medicine, Southern Medical University, Guangzhou, China, 510515.
Int J Med Sci. 2021 Jan 1;18(1):169-175. doi: 10.7150/ijms.49906. eCollection 2021.
Hepatocellular carcinoma (HCC) is known as the fifth most common cancer in the world for its poor prognosis. New diagnostic markers and treatments are urgent to discover. To evaluate the protein expression of Tropomyosin4 (TPM4) and investigate its prognostic value in HCC, we collected 110 patients with different degrees of HCC and 10 patients with normal hepatic tissues and performed immunohistochemistry. Western bot was used to evaluate the expression of TPM4 in three HCC cell lines (HepG2, Huh7, SMMC-7721) and normal liver cell line LO2, as well as 7 HCC tissues and 7 normal hepatic tissues. The results of TPM4 staining revealed that TPM4 expression in HCC was higher than that in normal hepatic tissues, which was positive in 51.8% (n=57) and negative in 48.2% (n=53) while in normal hepatic tissues positive staining was in 10% (n=1) and negative staining was in 90% (n=9) (=0.011). And the expression of TPM4 was related to pT status, grade and stage (<0.001, =0.015 and <0.001, respectively). Western blot results indicated that TPM4 was high expressed in HCC cell line and HCC tissues. In conclusion, we believe that TPM4 can be applied as a diagnostic and prognostic marker to assist the management of HCC.
肝细胞癌（HCC）因其预后不良而被称为全球第五大常见癌症。急需发现新的诊断标志物和治疗方法。为了评估原肌球蛋白 4（TPM4）的蛋白表达并研究其在 HCC 中的预后价值，我们收集了 110 例不同程度 HCC 患者和 10 例正常肝组织患者，并进行了免疫组织化学染色。Western blot 用于评估 TPM4 在三种 HCC 细胞系（HepG2、Huh7、SMMC-7721）和正常肝细胞系 LO2 中的表达，以及 7 例 HCC 组织和 7 例正常肝组织。TPM4 染色结果显示，TPM4 在 HCC 中的表达高于正常肝组织，阳性率为 51.8%（n=57），阴性率为 48.2%（n=53），而正常肝组织阳性染色率为 10%（n=1），阴性染色率为 90%（n=9）（=0.011）。并且 TPM4 的表达与 pT 状态、分级和分期有关（<0.001、=0.015 和 <0.001）。Western blot 结果表明 TPM4 在 HCC 细胞系和 HCC 组织中高表达。综上所述，我们认为 TPM4 可以作为诊断和预后标志物，辅助 HCC 的管理。
