Deems Amanda, Du Prey Michael, Dowd Scot E, McLaughlin Richard William
General Studies, Gateway Technical College, 3520-30th Avenue, Kenosha, WI, 53144, USA.
MR DNA (Molecular Research LP), Shallowater, TX, USA.
Curr Microbiol. 2021 Feb;78(2):522-527. doi: 10.1007/s00284-020-02320-9. Epub 2021 Jan 3.
Acinetobacter species are gram-negative, non-fermenting bacteria with coccobacilli morphology. The bacteria are found ubiquitously and have the ability to occupy niches which include environmental sites, animals, and humans. The original purpose of this study was to determine if painted turtles (Chrysemys picta) living in the wild in Western Wisconsin were colonized with carbapenem-resistant bacteria. Fecal samples from ten turtles were examined for carbapenem-resistant bacteria. None of the isolates were found to be carbapenem resistant by antimicrobial susceptibility testing. However, all the isolates were resistant to other β-lactams and chloramphenicol classes of antimicrobials. One isolate, Acinetobacter oleivorans strain PT8, was selected for additional characterization, including whole-genome sequencing (WGS). Strain PT8 is capable of degrading biodiesel, forming biofilms, and has a putative type 6 gene cluster. Finally, the taxonomic position of the available whole-genome sequences of 25 A. oleivorans genomes from purified isolates was determined.
不动杆菌属是革兰氏阴性、不发酵的细菌，呈球杆菌形态。这种细菌广泛存在，能够占据包括环境场所、动物和人类在内的生态位。本研究的最初目的是确定生活在威斯康星州西部野外的彩龟（彩龟属）是否被耐碳青霉烯类细菌定植。对10只乌龟的粪便样本进行了耐碳青霉烯类细菌检测。通过药敏试验，未发现分离株对碳青霉烯类耐药。然而，所有分离株均对其他β-内酰胺类和氯霉素类抗菌药物耐药。选择了一株分离株，即食油不动杆菌PT8菌株进行进一步鉴定，包括全基因组测序（WGS）。PT8菌株能够降解生物柴油、形成生物膜，并且具有一个假定的6型基因簇。最后，确定了来自纯化分离株的25个食油不动杆菌基因组的可用全基因组序列的分类地位。
