Coleman Andrea, Bialasiewicz Seweryn, Marsh Robyn L, Grahn Håkansson Eva, Cottrell Kyra, Wood Amanda, Jayasundara Nadeesha, Ware Robert S, Zaugg Julian, Sidjabat Hanna E, Adams Jasmyn, Ferguson Josephine, Brown Matthew, Roos Kristian, Cervin Anders
Children's Health Research Centre, The University of Queensland Centre for Clinical Research, South Brisbane, Australia.
Department of Surgery-Otolaryngology, Head and Neck Surgery, Townsville University Hospital, Townsville, Australia.
J Pediatric Infect Dis Soc. 2021 Apr 30;10(4):468-476. doi: 10.1093/jpids/piaa141.
We explored the nasal microbiota in Indigenous Australian children in relation to ear and nasal health.
In total, 103 Indigenous Australian children aged 2-7 years (mean 4.7 years) were recruited from 2 Queensland communities. Children's ears, nose, and throats were examined and upper respiratory tract (URT) swabs collected. Clinical histories were obtained from parents/medical records. URT microbiota were characterized using culturomics with Matrix assisted laser desorption ionization-time of flight mass spectrometry (MALDI-TOF MS) identification. Real-time PCR was used to quantify otopathogen (Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis) loads and detect respiratory viruses. Data were analyzed using beta diversity measures, regression modeling, and a correlation network analysis.
Children with historical/current otitis media (OM) or URT infection (URTI) had higher nasal otopathogen detection and loads and rhinovirus detection compared with healthy children (all P < .04). Children with purulent rhinorrhea had higher nasal otopathogen detection and loads and rhinovirus detection (P < .04) compared with healthy children. High otopathogen loads were correlated in children with historical/current OM or URTI, whereas Corynebacterium pseudodiphtheriticum and Dolosigranulum pigrum were correlated in healthy children.
Corynebacterium pseudodiphtheriticum and D. pigrum are associated with URT and ear health. The importance of the main otopathogens in URT disease/OM was confirmed, and their role relates to co-colonization and high otopathogens loads.
我们探讨了澳大利亚原住民儿童的鼻腔微生物群与耳和鼻健康的关系。
从昆士兰州的2个社区招募了总共103名2至7岁（平均4.7岁）的澳大利亚原住民儿童。对儿童的耳、鼻和喉进行检查，并采集上呼吸道（URT）拭子。从父母/医疗记录中获取临床病史。使用培养组学结合基质辅助激光解吸电离飞行时间质谱（MALDI-TOF MS）鉴定来表征URT微生物群。使用实时PCR定量耳病原体（流感嗜血杆菌、肺炎链球菌和卡他莫拉菌）载量并检测呼吸道病毒。使用β多样性测量、回归模型和相关网络分析对数据进行分析。
与健康儿童相比，有既往/当前中耳炎（OM）或上呼吸道感染（URTI）的儿童鼻腔耳病原体检测率和载量以及鼻病毒检测率更高（所有P <.04）。与健康儿童相比，有脓性鼻漏的儿童鼻腔耳病原体检测率和载量以及鼻病毒检测率更高（P <.04）。既往/当前患有OM或URTI的儿童中耳病原体载量相关，而健康儿童中假白喉棒状杆菌和嗜肉多尔氏菌相关。
假白喉棒状杆菌和嗜肉多尔氏菌与URT和耳健康有关。证实了主要耳病原体在上呼吸道疾病/OM中的重要性，其作用与共同定植和高耳病原体载量有关。
