School of Medicine, Zhejiang University City College, Hangzhou, Zhejiang, People's Republic of China.
General Hospital of NingXia Medical University, Yinchuan, Ningxia, People's Republic of China.
Mol Biol Rep. 2021 Jan;48(1):595-600. doi: 10.1007/s11033-020-06103-0. Epub 2021 Jan 4.
We previously reported that Numb, a protein localized to clathrin-coated vesicles, regulates the membrane expression of metabotropic glutamate receptor 5 (mGluR5) and is critical to social behaviors. However, the distinct actions of Numb isoforms on mGluR5 have not been investigated. Here, we showed that the expression patterns of Numb-p72 and Numb-p65, two important isoforms of Numb, were distinct in HEK293T cells. Numb-p72, but not Numb-p65, bound to mGluR5α, and enhanced mGluR5 membrane expression by inhibiting its internalization. Our results suggest that a complete structure is required for Numb to bind to mGluR5 and to modulate mGluR5 trafficking.
我们之前曾报道过，定位在网格蛋白包被小泡上的蛋白 Numb 调控代谢型谷氨酸受体 5（mGluR5）的膜表达，并且对社会行为至关重要。然而，Numb 同种型对 mGluR5 的不同作用尚未得到研究。在这里，我们表明，Numb-p72 和 Numb-p65 这两种 Numb 的重要同种型在 HEK293T 细胞中的表达模式不同。Numb-p72 而不是 Numb-p65 与 mGluR5α 结合，并通过抑制其内化来增强 mGluR5 的膜表达。我们的结果表明，Numb 与 mGluR5 结合并调节 mGluR5 运输需要完整的结构。
