Wojciak Gabriela, Szymura Jadwiga, Szygula Zbigniew, Gradek Joanna, Wiecek Magdalena
Faculty of Physical Education and Sport, University of Physical Education in Kraków, 31-571 Kraków, Poland.
Department of Clinical Rehabilitation, Faculty of Motor Rehabilitation, University of Physical Education in Kraków, 31-571 Kraków, Poland.
Antioxidants (Basel). 2020 Dec 31;10(1):37. doi: 10.3390/antiox10010037.
The activity of antioxidant enzymes and sirtuins (Sirt) decreases along with age, which is counteracted by aerobic training. Sirtuins increase antioxidant defence. Whole-body cryotherapy (WBC) increases total antioxidant capacity (TAC) in young men. The aim of our study was to assess the impact of 24 WBC treatments on the blood concentration of selected sirtuins and the level of antioxidant defence as well as oxidative stress index of training and non-training men depending on age.
The study involved 40 males. In each group, there were 10 non-training older and young men (60 NTR and 20 NTR), and 10 older and young long-distance runners (60 TR, 20 TR). During an 8-week period, participants underwent 24 WBC treatments (3 min -130 °C), which were performed three times a week (Monday, Wednesday, Friday). The concentrations of Sirt1, Sirt3, TAC, total oxidative status and the activity of superoxide dismutase (SOD), catalase and glutathione peroxidase (GPx) in the blood were determined before 1 WBC and after 1 WBC, 12 WBC and 24 WBC.
After 1 WBC, the activity of GPx and the concentration of Sirt1 and TAC in 60 TR and TAC in 60 NTR increased. After 12 WBC, the level of Sirt1 in 20 NTR and SOD in 20 TR increased. After 24 WBC, the level of Sirt1 increased in 60 TR and in 20 NTR, Sirt3 in 60 TR and SOD in 20 TR.
Cryogenic temperatures increase blood levels of Sirt1 and Sirt3 and systemic antioxidant defence in men, but the effect is dependent on age, level of performed physical activity and the number of applied treatments.
抗氧化酶和沉默调节蛋白（Sirt）的活性会随着年龄增长而降低，有氧运动训练可对此起到抵消作用。沉默调节蛋白可增强抗氧化防御能力。全身冷冻疗法（WBC）可提高年轻男性的总抗氧化能力（TAC）。我们研究的目的是评估24次全身冷冻疗法对不同年龄的训练和未训练男性血液中特定沉默调节蛋白浓度、抗氧化防御水平以及氧化应激指数的影响。
该研究纳入了40名男性。每组中有10名未训练的老年男性和年轻男性（60名未训练者，20名未训练者），以及10名老年长跑运动员和年轻长跑运动员（60名训练者，20名训练者）。在为期8周的时间里，参与者接受了24次全身冷冻疗法治疗（3分钟，-130°C），每周进行三次（周一、周三、周五）。在第1次全身冷冻疗法之前、第1次全身冷冻疗法之后、第12次全身冷冻疗法之后和第24次全身冷冻疗法之后，测定血液中Sirt1、Sirt3、总抗氧化能力、总氧化状态以及超氧化物歧化酶（SOD）、过氧化氢酶和谷胱甘肽过氧化物酶（GPx）的活性。
在第1次全身冷冻疗法之后，60名训练者的谷胱甘肽过氧化物酶活性、Sirt1浓度以及总抗氧化能力，以及60名未训练者的总抗氧化能力均有所增加。在第12次全身冷冻疗法之后，20名未训练者的Sirt1水平和20名训练者的超氧化物歧化酶水平有所增加。在第24次全身冷冻疗法之后，60名训练者和20名未训练者的Sirt1水平增加，60名训练者的Sirt3水平和20名训练者的超氧化物歧化酶水平增加。
低温可提高男性血液中Sirt1和Sirt3的水平以及全身抗氧化防御能力，但效果取决于年龄、体育活动水平和治疗次数。
