National Center for Biodefense and Infectious Diseases, School of Systems Biology, George Mason University, Manassas, VA 20110, USA.
Virus-Cell Interaction Section, HIV Dynamics and Replication Program, Center for Cancer Research, National Cancer Institute-Frederick, Frederick, MD 21702, USA.
Viruses. 2020 Dec 30;13(1):46. doi: 10.3390/v13010046.
P-selectin glycoprotein ligand-1 (PSGL-1) is a cell surface glycoprotein that binds to P-, E-, and L-selectins to mediate the tethering and rolling of immune cells on the surface of the endothelium for cell migration into inflamed tissues. PSGL-1 has been identified as an interferon-γ (INF-γ)-regulated factor that restricts HIV-1 infectivity, and has recently been found to possess broad-spectrum antiviral activities. Here we report that the expression of PSGL-1 in virus-producing cells impairs the incorporation of SARS-CoV and SARS-CoV-2 spike (S) glycoproteins into pseudovirions and blocks pseudovirus attachment and infection of target cells. These findings suggest that PSGL-1 may potentially inhibit coronavirus replication in PSGL-1 cells.
P 选择素糖蛋白配体-1（PSGL-1）是一种细胞表面糖蛋白，可与 P、E 和 L 选择素结合，介导免疫细胞在内皮表面的连接和滚动，以实现细胞向炎症组织的迁移。PSGL-1 已被确定为一种干扰素-γ（INF-γ）调节因子，可限制 HIV-1 的感染性，最近还发现其具有广谱抗病毒活性。在这里，我们报告在产生病毒的细胞中表达 PSGL-1 会损害 SARS-CoV 和 SARS-CoV-2 刺突（S）糖蛋白掺入假病毒颗粒，并阻止假病毒附着和靶细胞感染。这些发现表明 PSGL-1 可能在 PSGL-1 细胞中抑制冠状病毒复制。
