Division of Infectious Diseases, Cincinnati Children's Hospital Medical Center and University of Cincinnati, 3333 Burnet Avenue, Cincinnati, OH 45229, USA.
Viruses. 2022 Feb 26;14(3):478. doi: 10.3390/v14030478.
The assembly of HIV-1 particles is a concerted and dynamic process that takes place on the plasma membrane of infected cells. An abundance of recent discoveries has advanced our understanding of the complex sequence of events leading to HIV-1 particle assembly, budding, and release. Structural studies have illuminated key features of assembly and maturation, including the dramatic structural transition that occurs between the immature Gag lattice and the formation of the mature viral capsid core. The critical role of inositol hexakisphosphate (IP6) in the assembly of both the immature and mature Gag lattice has been elucidated. The structural basis for selective packaging of genomic RNA into virions has been revealed. This review will provide an overview of the HIV-1 assembly process, with a focus on recent advances in the field, and will point out areas where questions remain that can benefit from future investigation.
HIV-1 颗粒的组装是一个协同的动态过程，发生在感染细胞的质膜上。大量的最新发现提高了我们对导致 HIV-1 颗粒组装、出芽和释放的复杂事件序列的理解。结构研究阐明了组装和成熟的关键特征，包括在不成熟的 Gag 晶格和成熟的病毒衣壳核心形成之间发生的剧烈结构转变。已经阐明了肌醇六磷酸 (IP6) 在不成熟和成熟 Gag 晶格组装中的关键作用。基因组 RNA 选择性包装到病毒粒子中的结构基础已经揭示。这篇综述将概述 HIV-1 的组装过程，重点介绍该领域的最新进展，并指出仍存在一些问题，这些问题可以从未来的研究中受益。
