Hubei Key Laboratory of Environmental and Health Effects of Persistent Toxic Substances, Jianghan University, Wuhan 430056, China; Key Laboratory of Environmental Nanotechnology and Health Effects, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China.
Key Laboratory of Environmental Nanotechnology and Health Effects, Research Center for Eco-Environmental Sciences, Chinese Academy of Sciences, Beijing 100085, China; University of Chinese Academy of Sciences, Beijing 100049, China.
Sci Total Environ. 2020 May 15;717:137178. doi: 10.1016/j.scitotenv.2020.137178. Epub 2020 Feb 7.
Dressings coated with silver nanoparticle (AgNP) are widely used in the management of acute and chronic wounds. However, whether AgNP exerts toxicity on wound healing remains ambiguous. To demonstrate the effects of AgNP on wound healing, we precisely quantified the recovery speed of wound by taking advantage of the fin regeneration of zebrafish. This method also enabled assessment of the adverse effect of AgNP on various steps of wound healing in vivo. We revealed that AgNP treatment at the concentration of 2 μg/ml impaired fin regeneration when exposure was performed at the phases of epithelialization and the beginning of blastema formation. Cell proliferation of regenerative blastema was significantly decreased after AgNP exposure. But the canonical signals including Wingless/Integrated (Wnt), Notch and Fibroblast growth factor (Fgf) which play important roles in cell proliferation during fin regeneration were not modulated at 36 hours post amputation (hpa). Further study showed that AgNP impaired fin regeneration through declining amputation-induced ROS as early as epithelialized phase at 18 hpa, rather than inducing ROS generation. AgNP exposure also promoted recruitment of neutrophils in the early phase of wound healing, which suggests that this event dampened amputation-induced ROS. Overall, this study suggested that application of AgNP-coated dressings should be carefully considered at the beginning stage of wound healing.
银纳米颗粒（AgNP）涂层敷料广泛用于急性和慢性伤口的处理。然而，AgNP 是否对伤口愈合有潜在毒性仍然存在争议。为了证明 AgNP 对伤口愈合的影响，我们利用斑马鱼的鳍再生精确地量化了伤口的恢复速度。这种方法还可以评估 AgNP 在体内对伤口愈合各个阶段的不良影响。我们发现，当 AgNP 在上皮化阶段和胚基形成初期暴露时，浓度为 2μg/ml 的 AgNP 处理会损害鳍的再生。AgNP 暴露后，再生胚基的细胞增殖显著减少。但是，在截肢后 36 小时（hpa），包括在鳍再生过程中对细胞增殖起重要作用的 Wingless/Integrated（Wnt）、Notch 和 Fibroblast growth factor（Fgf）在内的经典信号并未被激活。进一步的研究表明，AgNP 通过在 18 hpa 的上皮化阶段就降低了截肢诱导的 ROS，从而损害了鳍的再生，而不是诱导 ROS 的产生。AgNP 暴露还促进了中性粒细胞在伤口愈合的早期募集，这表明这一事件抑制了截肢诱导的 ROS。总的来说，这项研究表明，在伤口愈合的早期阶段，应谨慎考虑使用 AgNP 涂层敷料。
