Chang W, Lim J G, Hellström I, Gentry L E
Department of Microbiology and Immunology, University of Washington, Seattle 98195.
J Virol. 1988 Mar;62(3):1080-3. doi: 10.1128/JVI.62.3.1080-1083.1988.
A synthetic peptide derived from vaccinia virus growth factor (VGF) was used as an immunogen to prepare antiserum able to immunoprecipitate native VGF from both vaccinia virus-infected cell lysate and cell-free medium. Pulse-chase, tunicamycin treatment, and carbohydrate trimming experiments revealed that VGF is synthesized as a 19-kilodalton (kDa) precursor which is rapidly modified to a high-mannose-type 22-kDa protein. This cell-associated form is further processed into a 25-kDa polypeptide which, after proteolytic cleavage, releases the mature VGF into the medium as a 22-kDa glycoprotein.
一种源自痘苗病毒生长因子（VGF）的合成肽被用作免疫原，以制备能够从痘苗病毒感染的细胞裂解物和无细胞培养基中免疫沉淀天然VGF的抗血清。脉冲追踪、衣霉素处理和碳水化合物修剪实验表明，VGF作为一种19千道尔顿（kDa）的前体合成，该前体迅速被修饰为高甘露糖型22-kDa蛋白。这种细胞相关形式进一步加工成25-kDa多肽，经过蛋白水解切割后，将成熟的VGF作为22-kDa糖蛋白释放到培养基中。
