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用于检测液体样本中可溶性人类白细胞抗原G5亚型（HLA-G5）的新型核酸适配体金（Au）纳米颗粒（AuNPs-AptHLA-G5-1和AuNPs-AptHLA-G5-2）。

Novel nucleic acid aptamer gold (Au)-nanoparticles (AuNPs-AptHLA-G5-1 and AuNPs-AptHLA-G5-2) to detect the soluble human leukocyte antigen G5 subtype (HLA-G5) in liquid samples.

作者信息

Su Tao, Wang Hui, Yao Yuanqing

机构信息

Medical School of Chinese PLA & Reproductive Center, the First Medical Center of Chinese PLA General Hospital, Beijing, China.

出版信息

Ann Transl Med. 2021 Sep;9(18):1416. doi: 10.21037/atm-21-3334.

DOI:10.21037/atm-21-3334
PMID:34733968
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC8506699/
Abstract

BACKGROUND

The human leukocyte antigen G5 subtype (HLA-G5) is a major histocompatibility complex (MHC) molecule that is selectively expressed at the maternal-foetal tissue interface and is required for the successful implantation of the fertilized embryo. It is critical to detect HLA-G5, especially HLA-G5 expression in embryo fluid, during embryo incubation and culture. However, the specificity and sensitivity of traditional ELISA methods to detect sHLA-G5 are insufficient. This work aimed to explore novel nucleic acid aptamer gold (Au)-nanoparticles to detect soluble HLA-G5 in liquid samples.

METHODS

Soluble HLA-G5 was obtained using a prokaryotic expression system, and two novel aptamers (HLA-G5-Apt1 and HLA-G5-Apt2) detecting HLA-G5 were screened by the Systematic Evolution of Ligands by Exponential Enrichment (SELEX) method. Small (10 nm) gold nanoparticles (AuNPs) were incubated with AptHLAs to form two novel nucleic acid aptamers: Au-nanoparticles (AuNPs-AptHLA-G5-1 and AuNPs-AptHLA-G5-2).

RESULTS

The results showed that AptHLA-G5-1 and AptHLA-G5-2 have a high affinity for HLA-G5 and can detect its presence in liquid samples. Using the colorimetric sensing method, AuNPs-AptHLA-G1 had a detection limit as low as 20 ng/mL (recovery range between 98.7% to 102.0%), while AuNPs-AptHLA-G2 had a detection limit as low as 20 ng/mL (recovery range between 98.9% to 103.6%).

CONCLUSIONS

Our work demonstrates that novel AuNPs are efficient detectors for HLA-G5 and are useful for diagnosis and treatment in the field of obstetrics-gynaecology.

摘要

背景

人类白细胞抗原G5亚型（HLA - G5）是一种主要组织相容性复合体（MHC）分子，在母胎组织界面选择性表达，是受精卵成功着床所必需的。在胚胎培养过程中检测HLA - G5，尤其是胚胎液中的HLA - G5表达至关重要。然而，传统酶联免疫吸附测定（ELISA）方法检测可溶性HLA - G5的特异性和灵敏度不足。本研究旨在探索新型核酸适配体金（Au）纳米颗粒以检测液体样本中的可溶性HLA - G5。

方法

使用原核表达系统获得可溶性HLA - G5，并通过指数富集配体系统进化（SELEX）方法筛选出两种检测HLA - G5的新型适配体（HLA - G5 - Apt1和HLA - G5 - Apt2）。将小尺寸（10 nm）的金纳米颗粒（AuNPs）与AptHLAs孵育，形成两种新型核酸适配体：金纳米颗粒（AuNPs - AptHLA - G5 - 1和AuNPs - AptHLA - G5 - 2）。

结果

结果表明，AptHLA - G5 - 1和AptHLA - G5 - 2对HLA - G5具有高亲和力，能够检测其在液体样本中的存在。采用比色传感方法，AuNPs - AptHLA - G1的检测限低至20 ng/mL（回收率范围在98.7%至102.0%之间），而AuNPs - AptHLA - G2的检测限低至20 ng/mL（回收率范围在98.9%至103.6%之间）。

结论

我们的研究表明，新型AuNPs是HLA - G5的高效检测剂，在妇产科领域的诊断和治疗中具有应用价值。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/3de148fe9595/atm-09-18-1416-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/7ee298b76f17/atm-09-18-1416-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/cf68e57c370f/atm-09-18-1416-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/6fa079703ebe/atm-09-18-1416-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/21f651d89869/atm-09-18-1416-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/3de148fe9595/atm-09-18-1416-f5.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/7ee298b76f17/atm-09-18-1416-f1.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/cf68e57c370f/atm-09-18-1416-f2.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/6fa079703ebe/atm-09-18-1416-f3.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/21f651d89869/atm-09-18-1416-f4.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/19ea/8506699/3de148fe9595/atm-09-18-1416-f5.jpg

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J Obstet Gynaecol Res. 2021 Apr;47(4):1232-1242. doi: 10.1111/jog.14630. Epub 2021 Jan 11.
2
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Mikrochim Acta. 2021 Jan 11;188(2):38. doi: 10.1007/s00604-020-04697-7.
3
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Front Oncol. 2020 Dec 23;10:597468. doi: 10.3389/fonc.2020.597468. eCollection 2020.
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5
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