Summerhayes I C, Bradley N J, Joyce A D, Malone P M
New England Deaconess Hospital, Department of Surgery, Boston, Massachusetts.
J Urol. 1988 Feb;139(2):405-9. doi: 10.1016/s0022-5347(17)42444-x.
The temporal and spatial regeneration of denuded mouse bladders was characterized using antibody markers to mucosal and submucosal elements in bladder tissue. Mechanical stripping of bladder mucosa resulted in a plane of cleavage in the submucosa leaving the muscle layers intact. Fully regenerated bladders were observed after 14 to 21 days although submucosal elements showed abnormal vacuolation. Implantation of immortalized epithelial cell line BBN3 into denuded bladders resulted in solid tumor formation while, in contrast, transplantation of MB331 showed cystic structures with no indication of invasion. Confirmation of the epithelial and implanted origin of cells lining the lumina of implanted bladders were shown using different keratin antibodies. The benign behavior of MB331 in vivo is suggestive of a cell line representing a preneoplastic stage in carcinogenesis and demonstrates an approach to assess the in vivo phenotype of cell parameters established in vitro.
利用针对膀胱组织中黏膜和黏膜下层成分的抗体标记物，对去上皮小鼠膀胱的时空再生进行了表征。膀胱黏膜的机械剥离导致黏膜下层出现一个分裂平面，肌肉层保持完整。14至21天后观察到膀胱完全再生，尽管黏膜下层成分显示出异常空泡化。将永生化上皮细胞系BBN3植入去上皮膀胱会导致实体瘤形成，而相比之下，MB331移植则显示出囊性结构，没有侵袭迹象。使用不同的角蛋白抗体证实了植入膀胱腔内衬细胞的上皮和植入来源。MB331在体内的良性行为表明该细胞系代表致癌过程中的癌前阶段，并展示了一种评估体外建立的细胞参数体内表型的方法。
