Khan S R, Shevock P N, Hackett R L
Department of Pathology, College of Medicine, University of Florida, Gainesville.
J Urol. 1988 Feb;139(2):418-22. doi: 10.1016/s0022-5347(17)42447-5.
Organic matrix of human calcium oxalate urinary stones was obtained by demineralizing with EDTA. Lipids were extracted from the EDTA-insoluble matrix by chloroform methanol treatment. The whole matrix and its total lipid extract were then incubated in a metastable solution of calcium oxalate and depletion of calcium and oxalate ions from the calcifying solution was determined. Results of our studies described here show that urinary calcium oxalate stone matrix and its total lipid contents were capable of binding calcium and oxalate ions and of catalysing calcium oxalate crystal formation from a metastable calcium oxalate solution.
通过用乙二胺四乙酸（EDTA）脱矿质获得人草酸钙尿结石的有机基质。通过氯仿甲醇处理从EDTA不溶性基质中提取脂质。然后将整个基质及其总脂质提取物在草酸钙的亚稳溶液中孵育，并测定钙化溶液中钙和草酸根离子的消耗。此处描述的我们的研究结果表明，尿草酸钙结石基质及其总脂质含量能够结合钙和草酸根离子，并能催化从亚稳草酸钙溶液中形成草酸钙晶体。
