高压液相色谱法同时测定人体生物体液中的氯喹和甲硝唑。
Simultaneous determination of chloroquine and metronidazole in human biological fluid by high pressure liquid chromatography.
作者信息
Okonkwo P O, Eta E I
机构信息
Department of Pharmacology and Therapeutics, College of Medicine, University of Nigeria Enugu Campus.
出版信息
Life Sci. 1988;42(5):539-45. doi: 10.1016/0024-3205(88)90095-1.
Chloroquine (CQ) and metronidazole (MZ) were measured in human urine and plasma by HPLC with UV detection. This method was used to analyse plasma levels in 4 African volunteers after an oral dose of 1000 mg CQ and 750 mg MZ, in a European on weekly prophylaxis of 500 mg CQ, and on 50 hospital urine samples. In the Africans peak plasma levels were over 1 microgram/ml and peak time was 1 1/2-2 hr. In the European plasma levels ranged from 0.58 to 0.36 microgram/ml. Over 80% of the urine samples contained CQ, MZ or both. The assay system was found flexible and economical for the therapeutic monitoring of these two important tropical drugs.
采用高效液相色谱-紫外检测法测定人尿液和血浆中的氯喹（CQ）和甲硝唑（MZ）。该方法用于分析4名非洲志愿者口服1000 mg CQ和750 mg MZ后的血浆水平，一名欧洲人每周预防性服用500 mg CQ后的血浆水平，以及50份医院尿液样本。在非洲人中，血浆峰值水平超过1微克/毫升，峰值时间为1.5至2小时。在欧洲人中，血浆水平在0.58至0.36微克/毫升之间。超过80%的尿液样本含有CQ、MZ或两者皆有。结果发现，该检测系统对于这两种重要的热带药物的治疗监测具有灵活性和经济性。
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