College of Chemistry & Chemical Engineering, Yangzhou University, Yangzhou 225002, P. R. China.
ACS Appl Mater Interfaces. 2021 Jan 20;13(2):3295-3302. doi: 10.1021/acsami.0c14946. Epub 2021 Jan 5.
In this work, a nanocomposite of Zr-trimesic acid MOF (MOF-808) with carbon nanotube (CNT) was synthesized through an in situ formation of MOF-808 on the activated CNT. The synthesized materials were characterized by powder X-ray diffraction, transmission electron microscopy, X-ray photoluminescence spectroscopy, Brunauer-Emmett-Teller, Fourier transform infrared spectroscopy, and Raman spectroscopy. The protein compatible nature with high surface area and electrocatalytic ability of MOF-808 was utilized to construct an immunosensor for ultra low-level detection of the ovarian cancer biomarker, carbohydrate antigen 125 (CA 125). The mutual benefit of each constituent of the MOF-808/CNT composite was capable of producing highly enhanced electrochemical properties. A glassy carbon electrode modified with MOF-808/CNT was used as a platform to fabricate a label-free electrochemical immunosensor. The antibody binding sites of MOF-808/CNT were enriched by functionalization with streptavidin. The immunosensor exhibited two linear determination ranges of 0.001-0.1 and 0.1-30 ng·mL, and the calculated limit of detection was 0.5 pg·mL (S/N 3). The immunosensor showed excellent reproducibility and selectivity. The patient serum sample analysis was cross-verified with the electrochemiluminescence method with a relative error of 105-110%.
在这项工作中，通过在活性碳纳米管上原位形成 MOF-808，合成了 Zr-均苯三甲酸 MOF（MOF-808）与碳纳米管（CNT）的纳米复合材料。通过粉末 X 射线衍射、透射电子显微镜、X 射线光致发光光谱、Brunauer-Emmett-Teller、傅里叶变换红外光谱和拉曼光谱对合成材料进行了表征。MOF-808 具有蛋白质相容性、高比表面积和电催化能力，利用其构建了用于超低水平检测卵巢癌生物标志物糖链抗原 125（CA 125）的免疫传感器。MOF-808/CNT 复合材料的每个成分的相互受益能够产生高度增强的电化学性能。用 MOF-808/CNT 修饰的玻碳电极被用作平台来制备无标记电化学免疫传感器。通过与链霉亲和素功能化来富集 MOF-808/CNT 的抗体结合位点。该免疫传感器具有两个线性测定范围，分别为 0.001-0.1 和 0.1-30 ng·mL，计算出的检测限为 0.5 pg·mL（S/N 3）。该免疫传感器表现出优异的重现性和选择性。用相对误差为 105-110%的电化学发光法对患者血清样品进行了交叉验证。
