Key Laboratory of Sensing Technology and Biomedical Instrument of Guangdong Province, School of Biomedical Engineering, Sun Yat-sen UniversityRINGGOLD, Guangzhou 510006, China.
ACS Appl Mater Interfaces. 2021 Jan 20;13(2):2165-2178. doi: 10.1021/acsami.0c15133. Epub 2021 Jan 5.
Oxidative damage to cells from metabolites at a wound site is one of the trickiest factors inhibiting tissue regeneration, especially with bulk damage. In addition, an excessive inflammatory reaction by the body at the wound site can make it even worse. How to scavenge the reactive oxygen species (ROS) produced from metabolism and inflammatory reactions has become a critical issue in tissue engineering. Here, we utilize the natural bioactive small molecules l-arginine and l-phenylalanine and the growth factor inositol to synthesize a branched poly(ester amide) (BPEA) to fabricate BPEA nanocapsules for vitamin E delivery at wound sites. BPEA nanocapsules loaded with vitamin E (BPEA@VE NCs) could protect cells from both extracellular and intracellular damage by scavenging ROS. Simultaneously, the inflammatory reaction could also be downregulated, benefiting from the introduction of l-arginine. Furthermore, the biodegradation products of BPEA are natural metabolites of the body, such as amino acids and growth factors, guaranteeing the biocompatibility of the BPEA@VE NCs. The protective ability of the BPEA@VE NCs was also investigated for accelerated wound healing. All the results indicate that the BPEA@VE NCs have promising potential for the modulation of the local microenvironment in tissue engineering for excellent antioxidative and anti-inflammatory properties.
细胞在伤口部位的代谢物产生的氧化损伤是抑制组织再生的最棘手因素之一，尤其是对于大块损伤。此外，机体在伤口部位的过度炎症反应会使情况变得更糟。如何清除代谢和炎症反应产生的活性氧（ROS）已成为组织工程中的一个关键问题。在这里，我们利用天然生物活性小分子 l-精氨酸和 l-苯丙氨酸以及生长因子肌醇来合成支化聚酯酰胺（BPEA），以制造用于在伤口部位递送维生素 E 的 BPEA 纳米胶囊。负载维生素 E 的 BPEA 纳米胶囊（BPEA@VE NCs）能够通过清除 ROS 来保护细胞免受细胞外和细胞内损伤。同时，由于引入了 l-精氨酸，炎症反应也可以被下调。此外，BPEA 的生物降解产物是氨基酸和生长因子等人体的天然代谢物，保证了 BPEA@VE NCs 的生物相容性。还研究了 BPEA@VE NCs 对加速伤口愈合的保护作用。所有结果均表明，BPEA@VE NCs 具有良好的抗氧化和抗炎特性，有望用于调节组织工程中的局部微环境。
