Takehisa S, Kanaya N, Rieger R
Department of Biology, Keio University, Yokohama, Japan.
Mutat Res. 1988 Feb;197(2):195-205. doi: 10.1016/0027-5107(88)90093-0.
Plant activation of promutagens was studied using Vicia faba S10 (in vitro activation) and the extracts prepared from promutagen-treated roots of Vicia faba (in vivo activation). The induction of sister-chromatid exchanges in Chinese hamster ovary cells was used as an endpoint to evaluate the cytogenetic effects of promutagens activated by Vicia faba. Cyclophosphamide and ethyl alcohol were activated both by Vicia S10 and by the Vicia extracts, and their activation resulted in an increase in SCEs. Benzo[a]pyrene, 2-aminofluorene, and maleic hydrazide were not activated. Aniline was activated, but without effect on the induction of SCEs. The activation capacity in vitro and in vivo of Vicia faba was not very pronounced, except for the activation of ethyl alcohol, when compared with that of rat-liver S9, and showed differences in activation for the 6 chemical agents tested.
利用蚕豆S10（体外活化）和经前诱变剂处理的蚕豆根制备的提取物（体内活化）研究了植物对前诱变剂的活化作用。以中国仓鼠卵巢细胞中姐妹染色单体交换的诱导作为终点，评估蚕豆活化的前诱变剂的细胞遗传学效应。环磷酰胺和乙醇可被蚕豆S10和蚕豆提取物活化，它们的活化导致姐妹染色单体交换增加。苯并[a]芘、2-氨基芴和马来酰肼未被活化。苯胺被活化，但对姐妹染色单体交换的诱导没有影响。与大鼠肝脏S9相比，蚕豆的体外和体内活化能力除了对乙醇的活化外不是很明显，并且在所测试的6种化学试剂的活化方面存在差异。
