Department of Cardiology, Affiliated Hospital of Jiangsu University, 212000, Zhenjiang, China.
Department of Pathology, Affiliated Hospital of Jiangsu University, 212000, Zhenjiang, China.
Biomed Pharmacother. 2021 Mar;135:111192. doi: 10.1016/j.biopha.2020.111192. Epub 2021 Jan 2.
Vascular calcification is a high incidence and high risk disease with increasing morbidity and high mortality, which is considered the consequence of smooth muscle cell transdifferentiation initiating the mechanism of accumulation of hydroxyl calcium phosphate. Vascular calcification is also thought to be strongly associated with poor outcomes in diabetes and chronic kidney disease. Numerous studies have been accomplished; however, the specific mechanism of the disease remains unclear. Development of the genome project enhanced the understanding of life science and has entered the post-genomic era resulting in a variety of omics techniques used in studies and a large amount of available data; thus, a new perspective on data analysis has been revealed. Omics has a broader perspective and is thus advantageous over a single pathway analysis in the study of complex vascular calcification mechanisms. This paper reviews in detail various omics studies including genomics, proteomics, transcriptomics, metabolomics and multiple group studies on vascular calcification. Advances and deficiencies in the use of omics to study vascular calcification are presented in a comprehensive view. We also review the methodology of the omics studies and omics data analysis and processing. In addition, the methodology and data processing presented here can be applied to other areas. An omics landscape perspective across the boundaries between genomics, transcriptomics, proteomics and metabolomics is used to examine the mechanisms of vascular calcification. The perspective combined with various technologies also provides a direction for the subsequent exploration of clinical significance.
血管钙化是一种发病率和风险都很高的疾病，发病率不断上升，死亡率高，被认为是平滑肌细胞转分化启动羟基磷灰石积累机制的结果。血管钙化也被认为与糖尿病和慢性肾病的不良预后密切相关。已经完成了许多研究；然而，该疾病的确切机制仍不清楚。基因组计划的发展增强了对生命科学的理解，并进入了后基因组时代，导致各种组学技术被用于研究和产生了大量可用数据；因此，数据分析呈现出一种新的视角。组学具有更广泛的视角，因此在研究复杂的血管钙化机制方面优于单一途径分析。本文详细回顾了各种组学研究，包括基因组学、蛋白质组学、转录组学、代谢组学和血管钙化的多组学研究。全面介绍了组学在研究血管钙化中的应用的进展和不足。我们还回顾了组学研究和组学数据分析和处理的方法。此外，这里提出的方法和数据处理也可以应用于其他领域。通过跨越基因组学、转录组学、蛋白质组学和代谢组学之间界限的组学全景视角来研究血管钙化的机制。这种视角结合了多种技术，也为后续探索临床意义提供了方向。
