Clinic for Urology, Paediatric Urology and Andrology, Justus-Liebig University of Giessen, Giessen, Germany,
Department of Urology, Technical University of Munich, Munich, Germany.
Urol Int. 2021;105(3-4):278-284. doi: 10.1159/000512370. Epub 2021 Jan 5.
A catheter allowing a release of antibacterial substances such as antiseptics into the bladder could be a new way of preventing biofilm formation and subsequent catheter-associated urinary tract infections.
Minimal inhibitory and bactericidal concentration (MIC/MBC) determinations in cation-adjusted Mueller-Hinton broth and artificial urine were performed for 4 antiseptics against 3 uropathogenic biofilm producers, Escherichia coli, Pseudomonas aeruginosa, and Proteus mirabilis. Furthermore, effects of octenidine and polyhexanide against catheter biofilm formation were determined by quantification of biofilm-producing bacteria.
Sodium hypochlorite showed MIC/MBC values between 200 and 800 mg/L for all strains tested. Triclosan was efficient against E. coli and P. mirabilis (MIC ≤2.98 mg/L) but ineffective against P. aeruginosa. Octenidine and polyhexanide showed antibacterial activity against all 3 species tested (MIC 1.95-7.8 and 3.9-31.25 mg/L). Both octenidine and polyhexanide were able to prevent biofilm formation on catheter segments in a concentration dependent manner. Furthermore, adding 250 mg/L of each biocide disrupted biofilms formed by E. coli and P. mirabilis, whereas even 500 mg/L was not sufficient to completely destroy P. aeruginosa biofilms.
Octenidine- and polyhexanide-containing antiseptics showed a broad effect against typical uropathogenic biofilm producers even in high dilutions. This study provides a basis for further investigation of the potential of octenidine and polyhexanide as prophylaxis or treatment of catheter biofilms.
能够将抗菌物质（如防腐剂）释放到膀胱中的导管可能是预防生物膜形成和随后发生导管相关性尿路感染的新方法。
使用阳离子调整的 Mueller-Hinton 肉汤和人工尿液对 4 种防腐剂针对 3 种尿路生物膜产生菌，即大肠杆菌、铜绿假单胞菌和奇异变形杆菌进行最小抑菌浓度（MIC）和杀菌浓度（MBC）测定。此外，通过定量生物膜产生菌来确定奥替尼定和聚己双胍对导管生物膜形成的影响。
次氯酸钠对所有测试菌株的 MIC/MBC 值在 200 至 800mg/L 之间。三氯生对大肠杆菌和奇异变形杆菌有效（MIC≤2.98mg/L），但对铜绿假单胞菌无效。奥替尼定和聚己双胍对所有 3 种测试的菌株均具有抗菌活性（MIC 为 1.95-7.8 和 3.9-31.25mg/L）。奥替尼定和聚己双胍均能够以浓度依赖的方式防止导管段上的生物膜形成。此外，添加 250mg/L 的每种杀生物剂即可破坏大肠杆菌和奇异变形杆菌形成的生物膜，而即使添加 500mg/L 也不足以完全破坏铜绿假单胞菌生物膜。
即使在高稀释度下，含奥替尼定和聚己双胍的防腐剂对典型的尿路生物膜产生菌也具有广泛的作用。本研究为进一步研究奥替尼定和聚己双胍作为导管生物膜的预防或治疗的潜力提供了依据。
