Department of Clinical Laboratory Sciences, College of Applied Medical Sciences, King Khalid University, Abha 61421, Saudi Arabia.
Department of Physiology, College of Medicine, King Khalid University, Abha 61421, Saudi Arabia.
Molecules. 2021 Jan 3;26(1):202. doi: 10.3390/molecules26010202.
The red-complex bacteria are one of the most significant complexes found simultaneously in subgingival plaque next to the periodontal pocket. The current antibacterial treatment is not adequate, and multidrug resistance to it is developing. Henceforth, the antibacterial effect of the ethanolic extract of was put to test against red-complex bacteria in patients with chronic periodontitis.
Well diffusion and micro broth dilution procedure by Alamar blue were applied to assess the zone of inhibition (ZOI), the minimum inhibitory concentration (MIC), and the minimum bactericidal concentration (MBC). Anti-virulence efficacies of the plant extract that comprise of adherence and formation of biofilms were examined by the process of adherence and biofilm production assay.
The crude extract of exhibited significant inhibitory outcome against periodontopathic bacteria with noteworthy MIC (0.78-3.12 mg/mL), inhibitory zone (12-20 mm), as well as MBC (3.12-12.50 mg/mL). The extract inhibited bacterial adhesion ranging from 41% to 52%, 53% to 66%, and 60% to 79% at the given MIC × 0.5, MIC × 1, and MIC × 2 in succession. Substantial suppression was also developed in the biofilm production of the investigated periodontopathic strains following exposure to numerous concentrations of extract for a period of 24 and 48 h.
These outcomes divulge a new concept that extract can be utilized to manufacture valuable antibacterial compounds to treat chronic and acute periodontitis. This identifies as an essential natural source for future drug development.
红色复合体细菌是牙周袋附近龈下菌斑中同时存在的最重要的复合体之一。目前的抗菌治疗并不充分，而且对其产生了多药耐药性。因此，本研究将测试 的乙醇提取物对慢性牙周炎患者的红色复合体细菌的抗菌作用。
采用琼脂扩散法和 Alamar blue 微量肉汤稀释法评估抑菌圈（ZOI）、最小抑菌浓度（MIC）和最小杀菌浓度（MBC）。采用黏附及生物膜形成试验检测植物提取物的抗毒力，评估其对黏附及生物膜形成的抑制作用。
的粗提物对牙周致病菌具有显著的抑制作用，MIC（0.78-3.12 mg/mL）、抑菌圈（12-20 mm）和 MBC（3.12-12.50 mg/mL）均显著。 在 MIC×0.5、MIC×1 和 MIC×2 依次作用下，对细菌黏附的抑制率分别为 41%-52%、53%-66%和 60%-79%。 提取物对所研究的牙周致病菌生物膜生成的抑制作用也很显著，在 24 和 48 h 作用不同浓度 提取物后，生物膜生成量明显减少。
这些结果揭示了一个新概念，即 提取物可用于制造有价值的抗菌化合物来治疗慢性和急性牙周炎。这表明 是未来药物开发的重要天然来源。
