Department of Medicinal Chemistry and Pharmacognosy, College of Pharmacy, Qassim University, Buraydah 51452, Saudi Arabia.
Department of Pharmacognosy, Faculty of Pharmacy, Al-Azhar University, Cairo 11371, Egypt.
Molecules. 2021 Jan 3;26(1):203. doi: 10.3390/molecules26010203.
is a medicinal herb that alleviates inflammations and fever. Chromatographic separation, phytochemical characterization, and in vitro biological activities of the plant -hexane extract were conducted for the first time in this study. Six compounds were isolated for the first time from the -hexane fraction of aerial parts and were identified on the bases of NMR and MS analyses as pseudo-taraxaterol (), pseudo-taraxasterol acetate (), 3β-acetoxytaraxaster-20-en-30-aldehyde (), calenduladiol-3--palmitate (), stigmasterol (), and α-tocospiro B (). Compound () was a rare tocopherol-related compound and was isolated for the first time from family Asteraceae, while compound () was isolated for the first time from genus . The total alcoholic extract and -hexane fraction were tested for their anti-inflammatory, antidiabetic, and cytotoxic activities. The -hexane fraction has dose dependent red blood cells (RBCs) membrane stabilization and inhibition of histamine release activities with IC: 60.8 and 72.9 µg/mL, respectively. As antidiabetic activity, the alcoholic extract exerted the most inhibition on the activity of yeast α-glucosidase, with an IC: 76.8 µg/mL. The -hexane fraction showed cytotoxic activity against hepatocarcinoma (HepG-2), breast carcinoma (MCF-7), and prostate carcinoma (PC-3) cell lines with IC: 51.8, 90.8 and 62.2 µg/mL, respectively. In conclusion, the anti-inflammatory effect of might be attributed to the triterpenoid constituents of the -hexane extract of the plant.
是一种草药，可缓解炎症和发热。本研究首次对该植物的正己烷提取物进行了色谱分离、植物化学特性分析和体外生物活性研究。首次从该植物的正己烷部分分离出 6 种化合物，并根据 NMR 和 MS 分析鉴定为伪蒲公英甾醇（）、伪蒲公英甾醇乙酸酯（）、3β-乙酰氧基蒲公英甾-20-烯-30-醛（）、筋骨草二醇-3--棕榈酸酯（）、豆甾醇（）和α-生育酚 B（）。化合物（）是一种罕见的生育酚相关化合物，首次从菊科中分离得到，而化合物（）则首次从属中分离得到。总醇提物和正己烷部分均进行了抗炎、抗糖尿病和细胞毒性活性测试。正己烷部分具有依赖于剂量的红细胞（RBC）膜稳定作用，并具有组胺释放抑制活性，IC: 60.8 和 72.9 µg/mL。作为抗糖尿病活性，醇提物对酵母α-葡萄糖苷酶的抑制作用最强，IC: 76.8 µg/mL。正己烷部分对肝癌（HepG-2）、乳腺癌（MCF-7）和前列腺癌（PC-3）细胞系表现出细胞毒性活性，IC: 51.8、90.8 和 62.2 µg/mL。综上所述，抗炎作用可能归因于该植物正己烷提取物中的三萜类成分。
