Boudebbaz Khadidja, Brouk Manel, Laalem Roumaissa, Zabaiou Nada
Laboratory of Pharmacology and Phytochemistry, Faculty of Exact Sciences and Informatics, University of Jijel, 18000, Jijel, Algeria.
Department of Chemistry, Faculty of Exact Sciences and Informatics, University of Jijel, 18000, Jijel, Algeria.
Heliyon. 2025 Jan 4;11(1):e41625. doi: 10.1016/j.heliyon.2025.e41625. eCollection 2025 Jan 15.
has been traditionally used in northeastern Algeria for treating gastrointestinal disorders, particularly ulcers. This study aimed to assess the gastroprotective, anti-inflammatory, and antioxidant properties of a crude hydroalcoholic extract derived from the leaves of , as well as its subsequent fractions. The gastroprotective effect was studied in an ethanol-induced ulcer model in mice. The antioxidant activity was quantified via the DPPH, and ABTS methods. anti-inflammatory activity was investigated through the human red blood cell membrane stabilization method and the bovine serum albumin denaturation method. The anti-inflammatory properties of the crude extract were assessed in mice via a carrageenan-induced acute paw edema model. The crude extract significantly inhibited gastric ulcer formation in a dose-dependent manner, achieving a reduction of up to 99.91 % at a dose of 200 mg/kg. Among the fractions, the aqueous fraction exhibited the most potent gastroprotective effect, with a notable dose-dependent response and high ulcer inhibition rates (95.56 % at 50 mg/kg). Additionally, the crude extract displayed strong and anti-inflammatory activity and a high free radical scavenging capacity; with IC50 values comparable to those of ascorbic acid. LC-ESI-MS analysis led to the identification of nine flavonoid derivatives, which were subsequently subjected to molecular docking techniques to predict their antiulcer activity. These results suggest that both glycosylated and aglycone flavonoids derived from have the potential to inhibit gastric acid secretion by targeting the proton pump.
在阿尔及利亚东北部，传统上一直使用[植物名称]来治疗胃肠道疾病，尤其是溃疡。本研究旨在评估从[植物名称]叶片中提取的粗制水醇提取物及其后续馏分的胃保护、抗炎和抗氧化特性。在乙醇诱导的小鼠溃疡模型中研究了胃保护作用。通过DPPH法和ABTS法对抗氧化活性进行了定量分析。通过人红细胞膜稳定法和牛血清白蛋白变性法研究了抗炎活性。通过角叉菜胶诱导的急性爪肿胀模型评估了粗提物在小鼠中的抗炎特性。粗提物以剂量依赖性方式显著抑制胃溃疡形成，在200mg/kg剂量下溃疡减少高达99.91%。在这些馏分中，水相馏分表现出最强的胃保护作用，具有显著的剂量依赖性反应和高溃疡抑制率（50mg/kg时为95.56%）。此外，粗提物表现出较强的抗炎活性和较高的自由基清除能力；其IC50值与抗坏血酸相当。液相色谱-电喷雾电离质谱分析鉴定出九种黄酮类衍生物，随后对其进行分子对接技术以预测其抗溃疡活性。这些结果表明，源自[植物名称]的糖基化和苷元黄酮类化合物都有可能通过靶向质子泵来抑制胃酸分泌。
