Okeke-Nwolisa B C, Enweani-Nwokelo I B, Unekwe P C, Egbuonu I
Department of Medical Laboratory Science, Faculty of Health Sciences and Technology, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria.
Department of Pharmacology, Faculty of Basic Clinical Sciences, Nnamdi Azikiwe University, Nnewi Campus, Nnewi, Nigeria.
Int J Health Sci (Qassim). 2023 May-Jun;17(3):26-32.
The study aimed to determine the anti-diarrheal and anti-inflammatory activities of hydroethanolic extract of ripe fruit pulp (HEAMP) on Wistar rats.
Fifty male Wistar rats were divided into five groups, each for the diarrhea and inflammatory phases. Groups A and B 10 mL/kg of distilled water and 2 mL of castor oil, Groups C, D, and E received 2 mL of castor oil + extract (150, 300, and 600 mg/kg). Groups A and B received 10 mL/kg of distilled water and 0.1 mL of egg white. Groups C, D, and E received the (0.1 mL) egg white and extract (150, 300, and 600 mg/kg). Data were analyzed using SPSS (25) using ANOVA followed by least significant difference, presented as mean ± standard error of the mean, and values considered significant at < 0.05.
The acute toxicity test of HEAMP is above 5000 mg/kg and rich in flavonoids, saponins, and proteins, with carbohydrates and tannins, and glycosides at moderate levels. Steroids, alkaloids, amino acids, and triterpenoids were absent. Furthermore, 30 min after diarrhea induction, the treated groups significantly increased ( = 0.012) in fecal count compared to Group A. Compared to Group B, the treated groups had a significant decrease in the fecal count at 60, 90, 120, and 150 min. At 30-min, there was a significant increase ( = 0.000) in paw-size in the treated groups compared to Group A. The treated groups had significantly lower edematous paw-size levels from 60 to 150 min compared to Group B.
The HEAMP had anti-diarrheal and anti-inflammatory properties and is safe for consumption.
本研究旨在确定成熟果实果肉水乙醇提取物（HEAMP）对Wistar大鼠的止泻和抗炎活性。
将50只雄性Wistar大鼠分为五组，每组分别用于腹泻和炎症阶段。A组和B组分别给予10 mL/kg蒸馏水和2 mL蓖麻油，C组、D组和E组给予2 mL蓖麻油+提取物（150、300和600 mg/kg）。A组和B组给予10 mL/kg蒸馏水和0.1 mL蛋清。C组、D组和E组给予（0.1 mL）蛋清和提取物（150、300和600 mg/kg）。使用SPSS（25）软件进行数据分析，采用方差分析，随后进行最小显著差检验，结果以平均值±平均标准误差表示，P<0.05的值被认为具有统计学意义。
HEAMP的急性毒性试验结果高于5000 mg/kg，富含黄酮类、皂苷类和蛋白质，碳水化合物和单宁含量适中，糖苷含量中等。不含类固醇、生物碱、氨基酸和三萜类化合物。此外，腹泻诱导30分钟后，与A组相比，治疗组的粪便计数显著增加（P = 0.012）。与B组相比，治疗组在60、90、120和150分钟时的粪便计数显著减少。在30分钟时，与A组相比，治疗组的爪大小显著增加（P = 0.000）。与B组相比，治疗组在60至150分钟时的爪水肿大小水平显著降低。
HEAMP具有止泻和抗炎特性，食用安全。
