Kujawa Joanna, Głodek Marta, Koter Izabela, Ośmiałowski Borys, Knozowska Katarzyna, Al-Gharabli Samer, Dumée Ludovic F, Kujawski Wojciech
Faculty of Chemistry, Nicolaus Copernicus University in Toruń, 7 Gagarina Street, 87-100 Toruń, Poland.
Pharmaceutical and Chemical Engineering Department, German-Jordanian University, Amman 11180, Jordan.
Materials (Basel). 2021 Jan 3;14(1):201. doi: 10.3390/ma14010201.
A highly effective method was developed to functionalize ceramic supports (AlO powders and membranes) using newly synthesized spacer molecules. The functionalized materials were subsequently utilized for lipase B enzyme immobilization. The objective is to systematically evaluate the impact of various spacer molecules grafted onto the alumina materials will affect both the immobilization of the enzymes and specific material surface properties, critical to enzymatic reactors performance. The enzyme loading was significantly improved for the supports modified with shorter spacer molecules, which possessed higher grafting effectiveness on the order of 90%. The specific enzyme activity was found to be much higher for samples functionalized with longer modifiers yielding excellent enantioselectivity >97%. However, the enantiomeric ratio of the immobilized lipase was slightly lower in the case of shorter spacer molecules.
开发了一种高效方法，使用新合成的间隔分子对陶瓷载体（氧化铝粉末和膜）进行功能化。随后将功能化材料用于固定化脂肪酶B。目的是系统评估接枝到氧化铝材料上的各种间隔分子对酶固定化和特定材料表面性质的影响，这对酶反应器性能至关重要。对于用较短间隔分子修饰的载体，酶负载量显著提高，其具有约90%的较高接枝效率。发现用较长修饰剂功能化的样品的比酶活性要高得多，对映体选择性>97%。然而，在较短间隔分子的情况下，固定化脂肪酶的对映体比例略低。
