Department of Veterinary Medicine, Agricultural College of Yanbian University, Yanji, P.R. China.
College of Life Science and Technology, Heilongjiang Bayi Agricultural University, Daqing, P.R. China.
In Vivo. 2021 Jan-Feb;35(1):249-257. doi: 10.21873/invivo.12253.
BACKGROUND/AIM: Curcumin is a polyphenol that exerts a variety of pharmacological activities and plays an anti-cancer role in many cancer cells. It was recently reported that gasdermin E (GSDME) is involved in the progression of pyroptosis.
HepG2 cells were treated with various concentrations of curcumin and cell viability was examined using MTT assay, apoptosis was analysed using flow cytometry, reactive oxygen species (ROS) levels using dihydroethidium, LDH release using an LDH cytotoxicity assay, and protein expression using western blot.
Curcumin increased the expression of the GSDME N-terminus and proteins involved in pyrolysis, promoted HspG2 cell pyrolysis and increased intracellular ROS levels. Moreover, inhibition of the production of intracellular ROS with n-acetylcysteine (NAC) improved the degree of apoptosis and pyrolysis induced by curcumin.
Curcumin induces HspG2 cell death by increasing apoptosis and pyroptosis, and ROS play a key role in this process. This study improves our understanding of the potential anti-cancer properties of curcumin in liver cancer.
背景/目的：姜黄素是一种多酚，具有多种药理活性，在许多癌细胞中发挥抗癌作用。最近有报道称，gasdermin E（GSDME）参与了细胞焦亡的进展。
用不同浓度的姜黄素处理 HepG2 细胞，用 MTT 法检测细胞活力，用流式细胞术分析细胞凋亡，用二氢乙啶检测活性氧（ROS）水平，用 LDH 细胞毒性测定法检测 LDH 释放，用 Western blot 检测蛋白表达。
姜黄素增加了 GSDME N 端和参与细胞焦亡的蛋白的表达，促进了 HspG2 细胞焦亡并增加了细胞内 ROS 水平。此外，用 N-乙酰半胱氨酸（NAC）抑制细胞内 ROS 的产生可改善姜黄素诱导的细胞凋亡和细胞焦亡程度。
姜黄素通过增加细胞凋亡和细胞焦亡诱导 HspG2 细胞死亡，ROS 在这一过程中起关键作用。本研究提高了我们对姜黄素在肝癌中潜在抗癌特性的理解。
