Donos Nikolaos, Kostopoulos Lambros, Tonetti Maurizio, Karring Thorkild, Lang Niklaus P
Eastman Dental Institute, UCL, Department of Periodontology, London, UK.
Clin Oral Implants Res. 2006 Aug;17(4):434-8. doi: 10.1111/j.1600-0501.2006.01260.x.
To evaluate the osteoinductive potential of deproteinized bovine bone mineral (DBBM) and an enamel matrix derivative (EMD) in the muscle of rats. Sixteen rats were used in this study. The animals were divided in three groups. Group A: a pouch was created in one of the pectoralis profundis muscles of the thorax of the rats and DBBM particles (Bio-Oss) were placed into the pouch. Healing: 60 days. Group B: a small pouch was created on both pectoralis profundis muscles at each side of the thorax midline. In one side, a mixture of EMD (Emdogain) mixed with DBBM was placed into one of the pouches, whereas in the contralateral side of the thorax the pouch was implanted with DBBM mixed with the propylene glycol alginate (PGA--carrier for enamel matrix proteins of EMD). Healing: 60 days. Group C: the same procedure as group B, but with a healing period of 120 days. Qualitative histological analysis of the results was performed. At 60 days, the histological appearance of the DBBM particles implanted alone was similar to that of the particles implanted together with EMD or PGA at both 60 and 120 days. The DBBM particles were encapsulated into a connective tissue stroma and an inflammatory infiltrate. At 120 days, the DBBM particles implanted together with EMD or PGA exhibited the presence of resorption lacunae in some cases. Intramuscular bone formation was not encountered in any group. The implantation of DBBM particles alone, combined with EMD or its carrier (PGA) failed to exhibit extraskeletal, bone-inductive properties.
评估脱蛋白牛骨矿物质（DBBM）和釉基质衍生物（EMD）在大鼠肌肉中的骨诱导潜力。本研究使用了16只大鼠。将动物分为三组。A组：在大鼠胸部的一块胸深肌中创建一个袋，并将DBBM颗粒（Bio - Oss）放入袋中。愈合时间：60天。B组：在胸部中线两侧的两块胸深肌上各创建一个小袋。在一侧，将EMD（Emdogain）与DBBM的混合物放入其中一个袋中，而在胸部的对侧，该袋植入与藻酸丙二醇酯（PGA - EMD釉基质蛋白的载体）混合的DBBM。愈合时间：60天。C组：与B组操作相同，但愈合期为120天。对结果进行了定性组织学分析。在60天时，单独植入的DBBM颗粒的组织学外观与在60天和120天时与EMD或PGA一起植入的颗粒相似。DBBM颗粒被包裹在结缔组织基质和炎性浸润中。在120天时，与EMD或PGA一起植入的DBBM颗粒在某些情况下出现了吸收腔。任何一组均未出现肌内骨形成。单独植入DBBM颗粒，与EMD或其载体（PGA）联合使用，均未表现出骨外骨诱导特性。
