Miron Richard J, Shuang Yuang, Sculean Anton, Buser Daniel, Chandad Fatiha, Zhang Yufeng
Faculté de Médecine Dentaire, Pavillon de Médecine Dentaire, Université Laval, Rue de la Terrasse, Québec, Canada.
Department of Periodontology, School of Dental Medicine, University of Bern, Freiburgstrasse 7, 3010, Bern, Switzerland.
Clin Oral Investig. 2016 Nov;20(8):2037-2043. doi: 10.1007/s00784-015-1702-2. Epub 2016 Jan 8.
The aim of the present study was to investigate the effects of Osteogain, a new formulation of enamel matrix derivative (EMD) in combination with a grafting material on a wide variety of genes for cytokines, transcription factors and extracellular matrix proteins involved in osteoblast differentiation.
Primary human periodontal ligament (PDL) cells were seeded on natural bone mineral (NBM) particles coated with Osteogain for 24 h and analyzed for regulated gene expression using a human osteogenesis gene super-array kit. Osteoblast-related genes include those transcribed during bone mineralization, ossification, bone metabolism, cell growth and differentiation as well as gene products representing extracellular matrix molecules, transcription factors and cell adhesion molecules.
Osteogain significantly upregulated the expression of over 20 of the 100 genes examined including bone morphogenetic protein 2 (BMP2), TGFβ1, fibroblast growth factor (FGF), epidermal growth factor (EGF) and platelet-derived growth factor (PDGF) as well as some of their associated receptors. Osteogain also promoted gene expression of a number of osteoblast differentiation markers including collagen1α2 and alkaline phosphatase as well as cell adhesion molecules including fibronectin and a variety of integrin binding proteins. Interestingly, Osteogain promoted calcitonin receptor 55-fold and also promoted annexin A5 gene expression over 12-fold.
The present study demonstrates that Osteogain is capable of either upregulating or downregulating the expression of a wide variety of genes including those for growth factors and cytokines when combined with a bone grafting material.
The results from the present study demonstrate the large and potent effect of addition of Osteogain in combination to a bone grafting material over a wide variety of genes supporting osteogenesis.
本研究旨在探究一种新型釉基质衍生物（EMD）配方Osteogain与一种移植材料联合使用对参与成骨细胞分化的多种细胞因子、转录因子和细胞外基质蛋白基因的影响。
将原代人牙周膜（PDL）细胞接种于涂有Osteogain的天然骨矿物质（NBM）颗粒上24小时，然后使用人类骨生成基因超级阵列试剂盒分析基因表达调控情况。成骨细胞相关基因包括在骨矿化、骨化、骨代谢、细胞生长和分化过程中转录的基因，以及代表细胞外基质分子、转录因子和细胞粘附分子的基因产物。
Osteogain显著上调了所检测的100个基因中的20多个基因的表达，包括骨形态发生蛋白2（BMP2）、转化生长因子β1（TGFβ1）、成纤维细胞生长因子（FGF）、表皮生长因子（EGF）和血小板衍生生长因子（PDGF）以及它们的一些相关受体。Osteogain还促进了许多成骨细胞分化标志物的基因表达，包括胶原蛋白1α2和碱性磷酸酶，以及细胞粘附分子，包括纤连蛋白和多种整合素结合蛋白。有趣的是，Osteogain使降钙素受体表达增加了55倍，还使膜联蛋白A5基因表达增加了12倍以上。
本研究表明，Osteogain与骨移植材料联合使用时，能够上调或下调多种基因的表达，包括生长因子和细胞因子的基因。
本研究结果表明，添加Osteogain与骨移植材料联合使用对多种支持骨生成的基因具有巨大而有效的影响。
