Department of Medical Chemistry Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.
Department of Pathophysiology Yerevan State Medical University after M. Heratsi, Yerevan, Armenia.
Noise Health. 2020 Jul-Sep;22(106):63-69. doi: 10.4103/nah.NAH_31_19.
Noise is one of the environmental factors, which is considered as a powerful stressor for the organism. Generally, the acoustic stress affects the behavior and physiological state of humans and animals.
The goal of this study is to investigate the relationship between chronic noise exposure and the effects of adrenergic alpha-2 receptor antagonists, beditin and mesedin, on the anxiety and oxidation of plasma proteins and fibrinogen in rats.
The experiments were carried out on non-linear albino male rats, divided into four groups (six animals in each): 1. Healthy controls 2. Exposed to noise of a level 91 dB(A), eight hours daily, during 7, 30 and 60 days; 3. Injected with 2 mg/kg of beditin (2-(2-amino-4-thiazolyl)-1,4-benzodioxane hydrochloride)); 4. Injected with 10 mg/kg mesedin (2-(2-methyl-amino-thiozolyl)-1,4-benzodioxane hydrochloride). For evaluating the cognitive impairment, the Any-maze test was applied. The level of carbonylation of proteins was assessed by reaction with 2,4-dinitrophenylhydrazine, spectrophotometrically.
Chronic noise decreased locomotor activity and increased anxiety and oxidation of plasma protein and fibrinogen. Intensity of these changes were dependent on the duration of noise exposure.
The Alpha 2 adrenoblockers alleviate oxidative modification of plasma proteins and reduce the cognitive impairment caused by chronic exposure to noise.
噪声是一种环境因素，被认为是生物体的一种强大应激源。通常，声应激会影响人类和动物的行为和生理状态。
本研究旨在探讨慢性噪声暴露与肾上腺素能 α2 受体拮抗剂苯丁氮酮和甲硫哒嗪对大鼠焦虑和血浆蛋白及纤维蛋白原氧化的影响之间的关系。
实验在非线性白化雄性大鼠上进行，分为四组（每组六只动物）：1. 健康对照组；2. 每天暴露于 91 dB(A) 水平的噪声中 8 小时，持续 7、30 和 60 天；3. 注射 2 mg/kg 的苯丁氮酮（2-(2-氨基-4-噻唑基)-1,4-苯并二恶烷盐酸盐）；4. 注射 10 mg/kg 的甲硫哒嗪（2-(2-甲基-氨基-噻唑基)-1,4-苯并二恶烷盐酸盐）。为了评估认知障碍，应用了 Any-maze 测试。通过与 2,4-二硝基苯肼反应，分光光度法评估蛋白质的羰基化水平。
慢性噪声降低了运动活动，增加了焦虑和血浆蛋白及纤维蛋白原的氧化。这些变化的强度取决于噪声暴露的持续时间。
α2 肾上腺素能阻滞剂减轻了血浆蛋白的氧化修饰，并减少了慢性暴露于噪声引起的认知障碍。
