Gozes I, Schächter P, Shani Y, Giladi E
Department of Hormone Research, Weizmann Institute of Science, Rehovot, Israel.
Neuroendocrinology. 1988 Jan;47(1):27-31. doi: 10.1159/000124886.
Vasoactive intestinal peptide (VIP) gene transcripts were demonstrated by RNA blot hybridization using VIP-specific RNA hybridization probes. High levels of expression were observed as early as in 16-day-old embryos. In aging rats, the VIP-mRNA levels were reduced significantly (in the cerebral cortex) as compared to 21-day-old rats. Our results suggest a role for the VIP gene protein products during embryonal development. During aging processes the decrease in VIP gene transcripts may be a consequence of either a reduction in the transcriptional activity of VIP neurons or death of VIP-producing cells.
使用血管活性肠肽（VIP）特异性RNA杂交探针，通过RNA印迹杂交法证实了VIP基因转录本的存在。早在16日龄胚胎中就观察到了高水平的表达。与21日龄大鼠相比，老龄大鼠（大脑皮层中）的VIP - mRNA水平显著降低。我们的结果表明VIP基因蛋白产物在胚胎发育过程中发挥作用。在衰老过程中，VIP基因转录本的减少可能是VIP神经元转录活性降低或产生VIP的细胞死亡的结果。
