Daei-Farshbaf Neda, Aflatoonian Reza, Amjadi Fatemeh-Sadat, Taleahmad Sara, Ashrafi Mahnaz, Bakhtiyari Mehrdad
Department of Anatomy, Faculty of Medicine, Iran University of Medical Sciences, Tehran Iran.
Department of Endocrinology and Female Infertility, Reproductive Biomedicine Research Center, Royan Institute for Reproductive Biomedicine, Academic Center for Education, Culture and Research, Tehran Iran.
Turk J Biol. 2020 Dec 14;44(6):371-380. doi: 10.3906/biy-2003-79. eCollection 2020.
Odorant or olfactory receptors are mainly localized in the olfactory epithelium for the perception of different odors. Interestingly, many ectopic olfactory receptors with low expression levels have recently been found in nonolfactory tissues to involve in local functions. Therefore, we investigated the probable role of the olfactory signaling pathway in the surrounding microenvironment of oocyte. This study included 22 women in intracytoplasmic sperm injection cycle. The expression of olfactory target molecules in cumulus cells surrounding the growing and mature oocytes was evaluated by Western blotting and real-time polymerase chain reaction. Additionally, integrated bioinformatics analyses were carried out and 6 ectopic olfactory receptors were selected for further evaluation. The initiation of olfactory transduction cascade in cumulus cells of competent oocytes was confirmed by analyzing the expression of adenylyl cyclase type 3 and olfactory market protein. Moreover, the expression pattern of the selected olfactory receptors was evaluated and OR10H2 was selected due to a high level of expression in mature fertile oocytes. We suggested that OR10H2 could be considered as a reliable biomarker for oocyte selection in assisted reproduction technique programs. However, further studies are required to elucidate the role of olfactory transduction cascade in embryo quality and implantation.
气味剂或嗅觉受体主要定位于嗅觉上皮，用于感知不同气味。有趣的是，最近在非嗅觉组织中发现了许多低表达水平的异位嗅觉受体，它们参与局部功能。因此，我们研究了嗅觉信号通路在卵母细胞周围微环境中的可能作用。本研究纳入了22名接受胞浆内单精子注射周期的女性。通过蛋白质免疫印迹法和实时聚合酶链反应评估生长中和成熟卵母细胞周围卵丘细胞中嗅觉靶分子的表达。此外，进行了综合生物信息学分析，并选择了6种异位嗅觉受体进行进一步评估。通过分析3型腺苷酸环化酶和嗅觉标记蛋白的表达，证实了有能力的卵母细胞的卵丘细胞中嗅觉转导级联反应的启动。此外，评估了所选嗅觉受体的表达模式，并由于其在成熟可育卵母细胞中的高表达水平而选择了OR10H2。我们认为，OR10H2可被视为辅助生殖技术程序中卵母细胞选择的可靠生物标志物。然而，需要进一步研究以阐明嗅觉转导级联反应在胚胎质量和着床中的作用。
