Department of Biomedical Science, Faculty of Health Sciences, University of Bamenda, Cameroon.
Department of Medical Laboratory Science, Faculty of Health Sciences, University of Bamenda, Cameroon.
Afr Health Sci. 2020 Sep;20(3):1045-1052. doi: 10.4314/ahs.v20i3.7.
HIV causes a decrease in CD4+ lymphocyte cells count, exposing the individual to infections (urinary tract infections). This study was carried out to determine the prevalence of bacteriuria and antimicrobial susceptibility pattern of bacteria isolates among HIV patients.
Clean catch mid-stream urine samples were collected from 135 HIV- seropositive patients, cultured on Cystein lactose electrolyte deficient (CLED) agar and incubated at 370C for 24 hours. The modified Kirby-Bauer's disc diffusion method was used to assess susceptibility to antimicrobial agents.
The prevalence of bacteriuria was 67.4% (91/135). was the most predominant (42.9%) isolate, followed by (24.2%), then Coagulase negative (10.9%). The highest proportion of bacteria was isolated from patients having a CD4+ T-cell count of less than 300 cells/mm3 (39.6%). There was an association between the level of CD4+cell count and bacterial urinary tract infection (P= 0.001). Most sensitive drugs were gentamycin, vancomycin and amoxicillin-clavulanic acid while the drug with the greatest resistance was sulphamethoxazole-trimethoprim, with and showing 100% resistance to this drug.
Bacteriuria and resistance to commonly used antibiotics is prevalent among HIV/AIDS patients attending the Bamenda Regional Hospital. Therapy based on antimicrobial susceptibility test is encouraged.
HIV 会导致 CD4+ 淋巴细胞计数减少，使个体易感染（尿路感染）。本研究旨在确定 HIV 患者中菌尿的患病率和细菌分离株的抗菌药物敏感性模式。
从 135 例 HIV 血清阳性患者中采集清洁中段尿样，接种于半胱氨酸乳糖电解质缺乏（CLED）琼脂上，并在 37°C 孵育 24 小时。采用改良 Kirby-Bauer 纸片扩散法评估抗菌药物敏感性。
菌尿的患病率为 67.4%（91/135）。最主要的分离株为 （42.9%），其次是 （24.2%），然后是凝固酶阴性 （10.9%）。CD4+T 细胞计数小于 300 个细胞/mm3 的患者中分离出的细菌比例最高（39.6%）。CD4+细胞计数水平与细菌尿路感染之间存在关联（P=0.001）。最敏感的药物是庆大霉素、万古霉素和阿莫西林克拉维酸，而最耐药的药物是磺胺甲噁唑-甲氧苄啶，对该药物的耐药率为 和 均为 100%。
班吉地区医院就诊的 HIV/AIDS 患者中存在菌尿和对常用抗生素的耐药性。鼓励根据抗菌药物敏感性试验进行治疗。
