Department of Pharmacy, College of Health Science, Assosa University, Assosa, Ethiopia.
Department of Pharmacology and Clinical Pharmacy, Addis Ababa University, Addis Ababa, Ethiopia.
BMC Microbiol. 2021 Dec 16;21(1):348. doi: 10.1186/s12866-021-02417-6.
Asymptomatic bacteriuria is a common problem in pregnant women and about 40% of women with untreated asymptomatic bacteriuria during pregnancy develop pyelonephritis, which might lead to low birth weight, premature rupture of membranes, and preterm labour. Therefore, this study aimed to assess the prevalence of asymptomatic bacteriuria, antimicrobial susceptibility pattern of the isolates among pregnant women attending the antenatal care of Assosa general hospital, western Ethiopia.
A facility-based cross-sectional study was conducted from January to February 2019. Two hundred and eighty-three pregnant women with no symptoms of urinary tract infections participated in the study. Bacterial isolates were identified as per the standard bacteriological procedure using colony characteristics, Gram-staining, and series of biochemical tests. Antimicrobial susceptibility test was carried out by Kirby- Bauer disk diffusion technique on Muller-Hinton agar medium and the diameter of zone of inhibition was interpreted according to Clinical Laboratory Standard Institute guidelines.
The overall prevalence of asymptomatic bacteriuria among pregnant women in this study was 13.78% (i.e. 39 out of 283 urine samples were positive for bacterial isolates). E. coli was the most predominant isolate (53.8%) followed by K. pneumoniae (17.95%), S. aureus (15.4%), and coagulase-negative staphylococci (12.8%). Gram-negative bacteria were highly resistant to tetracycline (96.4%), and ampicillin (90.5%).
Significant bacteriuria was observed in asymptomatic pregnant women. A large number of the bacterial isolates were resistant to the commonly used antimicrobial drugs.
无症状菌尿是孕妇中常见的问题，约有 40%未经治疗的无症状菌尿孕妇会发展为肾盂肾炎，这可能导致低出生体重、胎膜早破和早产。因此，本研究旨在评估无症状菌尿的患病率，以及埃塞俄比亚西部阿索萨综合医院产前护理孕妇中分离株的抗菌药物敏感性模式。
这是一项 2019 年 1 月至 2 月进行的基于机构的横断面研究。283 名无症状尿路感染孕妇参加了这项研究。细菌分离物根据标准细菌学程序，通过菌落特征、革兰氏染色和一系列生化试验进行鉴定。抗菌药物敏感性试验采用 Kirby-Bauer 纸片扩散技术在 Muller-Hinton 琼脂培养基上进行，根据临床实验室标准协会指南解释抑菌环直径。
本研究中孕妇无症状菌尿的总体患病率为 13.78%（即 283 份尿液样本中有 39 份呈细菌分离物阳性）。大肠杆菌是最主要的分离株（53.8%），其次是肺炎克雷伯菌（17.95%）、金黄色葡萄球菌（15.4%）和凝固酶阴性葡萄球菌（12.8%）。革兰氏阴性菌对四环素（96.4%）和氨苄西林（90.5%）高度耐药。
无症状孕妇中存在明显菌尿。大量细菌分离株对常用抗菌药物有耐药性。
