豚鼠脑中胆碱乙酰转移酶的免疫组织化学

Immunohistochemistry of choline acetyltransferase in the guinea pig brain.

作者信息

Maley B E, Frick M L, Levey A I, Wainer B H, Elde R P

机构信息

Department of Anatomy and Neurobiology, Chandler Medical Center, University of Kentucky, Lexington 40536.

出版信息

Neurosci Lett. 1988 Jan 22;84(2):137-42. doi: 10.1016/0304-3940(88)90397-7.

DOI:10.1016/0304-3940(88)90397-7

PMID:3340318

Abstract

Choline acetyltransferase (ChAT) was localized immunohistochemically within the brain of the guinea pig using a monoclonal antibody. ChAT was found in the cytoplasm of cell bodies and primary dendrites of neurons located in striatum, basal forebrain, cranial nerve motor nuclei and scattered cells in the pons. The greatest numbers of immunoreactive neurons were located in the diagonal band of Broca, medial septum and striatum. Distinct immunoreactive fibers were not visible using this antibody, although a diffuse immunostaining was present in the same nuclear regions as well as in the nerve roots of cranial nerve nuclei and the interpeduncular nuclei. Results of the present study agree closely with other previous reports of acetylcholine distributions.

摘要

使用单克隆抗体，通过免疫组织化学方法在豚鼠脑内定位胆碱乙酰转移酶（ChAT）。在位于纹状体、基底前脑、脑神经运动核以及脑桥中散在细胞的神经元胞体和初级树突的细胞质中发现了ChAT。免疫反应性神经元数量最多的位于布罗卡斜带、内侧隔区和纹状体。使用该抗体未观察到明显的免疫反应性纤维，尽管在相同的核区域以及脑神经核神经根和脚间核中存在弥漫性免疫染色。本研究结果与先前关于乙酰胆碱分布的其他报道密切一致。