Hoi Julia K, Lieder Barbara, Liebisch Beatrix, Czech Christiane, Hans Joachim, Ley Jakob P, Somoza Veronika
Department of Physiological Chemistry, Faculty of Chemistry, University of Vienna, Althanstraße 14, 1300 Vienna, Austria.
Symrise AG, Muehlenfeldstraße 1, 37603 Holzminden, Germany.
ACS Omega. 2020 Dec 15;5(51):33305-33313. doi: 10.1021/acsomega.0c05083. eCollection 2020 Dec 29.
The cinnamon-derived bioactive aroma compound cinnamaldehyde (CAL) has been identified as a promising antiobesity agent, inhibiting adipogenesis and decreasing lipid accumulation in vitro as well as in animal models. Here, we investigated the antiadipogenic effect of cinnamyl isobutyrate (CIB), another cinnamon-derived aroma compound, in comparison to CAL in 3T3-L1 adipocyte cells. In a concentration of 30 μM, CIB reduced triglyceride (TG) and phospholipid (PL) accumulation in 3T3-L1 pre-adipocytes by 21.4 ± 2.56 and 20.7 ± 2.05%, respectively. CAL (30 μM), in comparison, decreased TG accumulation by 37.5 ± 1.81% and PL accumulation by 28.7 ± 1.83%, revealing the aldehyde to be the more potent antiadipogenic compound. The CIB- and CAL-mediated inhibition of lipid accumulation was accompanied by downregulation of essential adipogenic transcription factors PPARγ, C/EBPα, and C/EBPβ on gene and protein levels, pointing to a compound-modulated effect on adipogenic signaling cascades. Coincubation experiments applying the TRPA-1 inhibitor AP-18 demonstrated TRPA1 dependency of the CAL, but not the CIB-induced antiadipogenic effect.
源自肉桂的生物活性香气化合物肉桂醛（CAL）已被确认为一种有前景的抗肥胖剂，在体外以及动物模型中均能抑制脂肪生成并减少脂质积累。在此，我们研究了异丁酸肉桂酯（CIB）——另一种源自肉桂的香气化合物——与CAL相比在3T3-L1脂肪细胞中的抗脂肪生成作用。在30 μM的浓度下，CIB使3T3-L1前脂肪细胞中的甘油三酯（TG）和磷脂（PL）积累分别减少了21.4 ± 2.56%和20.7 ± 2.05%。相比之下，CAL（30 μM）使TG积累减少了37.5 ± 1.81%，PL积累减少了28.7 ± 1.83%，表明醛类是更有效的抗脂肪生成化合物。CIB和CAL介导的脂质积累抑制伴随着关键脂肪生成转录因子PPARγ、C/EBPα和C/EBPβ在基因和蛋白质水平的下调，表明化合物对脂肪生成信号级联有调节作用。应用TRPA-1抑制剂AP-18的共孵育实验表明，CAL诱导的抗脂肪生成作用依赖于TRPA1，而CIB诱导的抗脂肪生成作用则不依赖于TRPA1。
