Šínová Romana, Žádníková Petra, Šafránková Barbora, Nešporová Kristina
Contipro a.s., Dolni Dobrouc 401, 561 02 Dolni Dobrouc, Czech Republic.
Department of Experimental Biology, Faculty of Science, Masaryk University, Kamenice 753/5, 625 00 Brno, Czech Republic.
Glycobiology. 2021 Jun 3;31(5):520-523. doi: 10.1093/glycob/cwaa118.
It is generally known that hyaluronic acid (HA) is a biocompatible and biodegradable glycosaminoglycan distributed widely throughout epithelial, connective and neural tissues. HA is one of the chief components of the extracellular matrix. Lack of immunogenicity is one of the biggest advantages of the therapeutic use of HA, but it also prevents the production of specific anti-HA antibodies. Contrary to this, there are still several studies performing HA detection by immunohistochemical or immunohistofluorescent method using an anti-HA antibody. Therefore, this short study discusses whether the anti-HA antibody is specific for HA. To verify the specificity of the HA staining the hyaluronidase treatment of histological samples was performed and the ability of anti-HA antibody and biotinylated HA binding protein (bHABP)-based probe to bind to their targets was evaluated. Additionally, the competitive binding assay with short HA oligosaccharides and subsequent histological staining was performed. Both assays showed that the anti-HA antibody is not sufficiently specific for HA and that the bHABP probe is a reliable method for HA detection in histological samples. The conclusion made by previous investigators based on using HA antibodies should be reevaluated and future use of anti-HA antibody should be avoided.
众所周知，透明质酸（HA）是一种生物相容性和可生物降解的糖胺聚糖，广泛分布于上皮组织、结缔组织和神经组织中。HA是细胞外基质的主要成分之一。缺乏免疫原性是HA治疗应用的最大优势之一，但这也阻碍了特异性抗HA抗体的产生。与此相反，仍有多项研究使用抗HA抗体通过免疫组织化学或免疫荧光方法进行HA检测。因此，这项简短的研究探讨了抗HA抗体是否对HA具有特异性。为了验证HA染色的特异性，对组织学样本进行了透明质酸酶处理，并评估了抗HA抗体和基于生物素化HA结合蛋白（bHABP）的探针与它们靶标的结合能力。此外，进行了短链HA寡糖的竞争性结合试验及随后的组织学染色。两项试验均表明，抗HA抗体对HA的特异性不足，而bHABP探针是在组织学样本中检测HA的可靠方法。基于使用HA抗体得出的先前研究者的结论应重新评估，并且应避免未来使用抗HA抗体。
