Department of Applied Sciences, University of Technology, Baghdad, Iraq.
College of Dentistry, Mustansiriyah University, Baghdad, Iraq.
Artif Cells Nanomed Biotechnol. 2021 Dec;49(1):48-60. doi: 10.1080/21691401.2020.1867152.
Nanoparticles (NPs) have biological activities like antibacterial, antifungal, drug delivery, immunomodulation and antitumor activities. The aim of the current study was to investigate some of biomedical applications of silver NP synthesis using extracts from leaves of . Colour changes, UV-visible spectroscopy, SEM, zeta potential, dynamic light scattering, FTIR and XRD were used to confirm AgNPs formation. The UV-vis spectrum absorption band was observed at almost 430 nm. The SEM image shows quasi-spherical shape of AgNPs. The zeta potential demonstrated the negative surface charge of NPs. FTIR results showed the functional groups of AgNPs. Crystalline nature of AgNPs was confirmed by XRD pattern. MTT assay was used to study the anti-proliferative activity against MCF-7 and HeLa cells. Apoptosis was tested using a DNA-fragmentation test, and expression of P. AgNPs inhibited the proliferation of MCF-7 and HeLa cells, and reduced inflammation. Treatment with AgNPs significantly decreased allergic disorder. AgNPs stimulated the phagocytosis process in BMDMs. The results suggested that AgNPs could be a promising therapy for future and preventing inflammation, reduce allergic disorders and prevent bacterial infection through the up-regulation of phagocytosis. Hence, future work such as developed and improved NPs as adjuvants, immune-modulating substances and nano-drug delivery system is needed.
纳米粒子（NPs）具有抗菌、抗真菌、药物传递、免疫调节和抗肿瘤等生物活性。本研究的目的是研究利用 叶片提取物合成银 NP 的一些生物医学应用。颜色变化、紫外-可见光谱、SEM、Zeta 电位、动态光散射、FTIR 和 XRD 用于证实 AgNPs 的形成。紫外可见光谱吸收带在近 430nm 处观察到。SEM 图像显示 AgNPs 的准球形形状。Zeta 电位表明 NPs 具有负表面电荷。FTIR 结果表明 AgNPs 的功能基团。XRD 图谱证实了 AgNPs 的结晶性质。MTT 测定法用于研究对 MCF-7 和 HeLa 细胞的抗增殖活性。使用 DNA 片段化试验测试细胞凋亡，并用 AgNPs 处理可抑制 MCF-7 和 HeLa 细胞的增殖，减少炎症。AgNPs 治疗可显著降低过敏症。AgNPs 刺激 BMDMs 的吞噬作用。结果表明，AgNPs 可能是未来治疗炎症、减少过敏症和预防细菌感染的有前途的方法，通过上调吞噬作用。因此，需要进行未来的工作，如开发和改进 NPs 作为佐剂、免疫调节物质和纳米药物传递系统。
