Green Synthesis of Characterized Silver Nanoparticle Using and Assessment of Its Antibacterial Activity.

Plants serve as an important source of medicine and provide suitable candidate compounds to produce eco-friendly therapeutic agents. They also represent a source of bio-reducer and stabilizer for the development of nanoparticles for downstream applications. This study focused on the green synthesis of silver nanoparticle (CTAgNP) using (Thunb.) J.W. Grimes acetone extract and the evaluation of the antibacterial activity of the plant extract and biogenic nanoparticles against two Gram-positive bacteria strains, namely and . In addition, the phytochemical profile of . was established using liquid chromatography-mass spectrometry (LC-MS). The antibacterial effect of the extract and CTAgNP was moderate based on the minimum inhibitory concentration (MIC) values obtained. The MIC values of 2.6 mg/mL and 3.1 mg/mL were recorded for . extract against . and . , respectively. On the other hand, the CTAgNP had MIC values of 1.5 mg/mL and 2.6 mg/mL against and , respectively. The nanoparticle exhibited surface charge of -37 ± 7.67 mV and average hydro-dynamic size of 145 nm. X-ray diffraction illustrates that metallic nanoparticles were formed and had a face-centered cubic structure. Microscopic and spectroscopic techniques revealed that the CTAgNP was covered by a protective shell layer constituted of organic compounds originating from the plant extract. The acetone extract of . could be useful to the bio-pharma industries in the large-scale manufacture of nanoparticle-based medications to fight against microbes that constitute a threat to the survival of humanity.