Faculty of Biology, Universitas Nasional, Jakarta, Indonesia.
Faculty of Biotechnology, Atma Jaya Catholic University of Indonesia, Jakarta, Indonesia.
Pharm Biol. 2021 Dec;59(1):31-39. doi: 10.1080/13880209.2020.1865408.
Linn. (Pandanaceae) seed extract is known to have antioxidant activities. However, the potential hepatoprotective effect is still unclear.
To investigate the hepatoprotection aspect of methanol extract towards paracetamol-induced rats.
Thirty male Sprague-Dawley rats were randomly divided into six equal groups: one group served as the healthy control and five groups with hepatotoxicity (hepatotoxic control and 4 treatment groups). The oral treatment of paracetamol-induced hepatotoxicity of 3 g/kg using three different concentrations of (300, 600 and 900 mg/kg), and silymarin (200 mg/kg) groups were administered once a day for 14 days. Enzyme activities and protein levels in serum were determined in rats at the end of the treatments. The histopathology of rat livers was observed under an electron microscope with 10× magnification.
significantly decreased the serum glutamic-oxaloacetic transaminase (SGOT), serum glutamic pyruvic transaminase (SGPT), alkaline phosphatase (ALP) and γ-glutamyl transferase (GGT) activities in induced-paracetamol rat serum ( < 0.05). Moreover, significantly decreased total bilirubin and direct bilirubin levels ( < 0.05). It significantly blocked the decline of serum albumin and protein levels ( < 0.05). Histopathological changes amplified paracetamol-induced liver damage and the hepatoprotective effect of in the liver.
improved the hepatoprotective effect in a concentration-dependent manner by reducing related hepatic enzyme and protein markers, suggesting as a useful agent in hepatotoxicity treatment, and it can be generalized to a broader study population in different hepatotoxic animal models.
林氏（露兜树科）种子提取物具有抗氧化活性。然而，其潜在的保肝作用尚不清楚。
研究甲醇提取物对扑热息痛诱导大鼠的保肝作用。
30 只雄性斯普拉格-道利大鼠随机分为 6 组：一组为健康对照组，五组为肝毒性组（肝毒性对照组和 4 个治疗组）。使用三种不同浓度的（300、600 和 900mg/kg）和水飞蓟素（200mg/kg）对扑热息痛诱导的肝毒性大鼠进行口服治疗，每天一次，共 14 天。在治疗结束时测定大鼠血清中酶活性和蛋白水平。用 10×放大倍数的电子显微镜观察大鼠肝脏的组织病理学。
显著降低了诱导扑热息痛大鼠血清中谷草转氨酶（SGOT）、谷丙转氨酶（SGPT）、碱性磷酸酶（ALP）和γ-谷氨酰转移酶（GGT）的活性（<0.05）。此外，还显著降低了总胆红素和直接胆红素的水平（<0.05）。显著阻止了血清白蛋白和蛋白水平的下降（<0.05）。组织病理学变化放大了扑热息痛诱导的肝损伤，以及 对肝的保护作用。
以浓度依赖的方式改善了保肝作用，降低了相关的肝酶和蛋白标志物，表明 可作为肝毒性治疗的有效药物，可推广至不同肝毒性动物模型的更广泛研究人群。
