Akinboboye Tunbosun Emmanuel, Olaniyi Temitope Deborah, Adeleke Gbadebo E, Aborisade Abiodun Bukunmi, Adetutu Adewale
Department of Biochemistry, Ladoke Akintola University of Technology Ogbomoso, Oyo State, Nigeria.
Department of Medical Biochemistry, Osun State University Osun State, Nigeria.
Int J Biochem Mol Biol. 2025 Apr 15;16(1):1-15. doi: 10.62347/CLHF2294. eCollection 2025.
Paracetamol is a widely used over-the-counter drug for pain relief and fever management. However, its misuse through chronic overuse or acute overdose presents significant risks to human health, primarily causing hepatotoxicity and systemic oxidative stress.
This study evaluated the hepatoprotective, antioxidant, and anti-inflammatory effects of aqueous leaf extracts of and in mitigating paracetamol-induced liver damage in male Wistar rats.
Paracetamol administration (150 mg/kg) significantly elevated liver function markers (ALT, AST, ALP, and bilirubin), oxidative stress parameters (MDA), and inflammatory cytokines (IL-6 and TNF-α), while depleting antioxidant defenses (SOD and GSH). Disrupted lipid profiles were also observed in the paracetamol-only group. Pretreatment with Celosia trigyna and Euphorbia hirta extracts (125 mg/kg and 250 mg/kg) effectively ameliorated these effects by normalizing liver function markers, reducing oxidative stress and inflammation, and restoring lipid profiles. Molecular docking identified bioactive compounds such as rutin, quercetin, and kaempferol as potent inhibitors of Glutathione-S-Transferase, Tumor Necrosis Factor-alpha, and Cytochrome P450, with binding affinities of -9.3, -7.2, and -8.3 kcal/mol, respectively. These interactions underpin the antioxidant and anti-inflammatory activities observed .
These findings suggest that and have the potential to serve as natural prophylactic or therapeutic agents for mitigating paracetamol toxicity. Further research is required to isolate their active compounds and explore their synergistic potential with conventional treatments. This study bridges traditional medicine and modern pharmacology, offering innovative approaches to managing drug-induced liver.
对乙酰氨基酚是一种广泛使用的非处方药物，用于缓解疼痛和控制发热。然而，通过长期过度使用或急性过量使用对其进行滥用会对人类健康构成重大风险，主要会导致肝毒性和全身性氧化应激。
本研究评估了鸡冠花和大戟叶水提取物对减轻雄性Wistar大鼠对乙酰氨基酚诱导的肝损伤的保肝、抗氧化和抗炎作用。
给予对乙酰氨基酚（150毫克/千克）显著提高了肝功能指标（谷丙转氨酶、谷草转氨酶、碱性磷酸酶和胆红素）、氧化应激参数（丙二醛）和炎性细胞因子（白细胞介素-6和肿瘤坏死因子-α），同时消耗了抗氧化防御物质（超氧化物歧化酶和谷胱甘肽）。仅使用对乙酰氨基酚的组中还观察到脂质谱紊乱。用鸡冠花和大戟提取物（125毫克/千克和250毫克/千克）预处理可通过使肝功能指标正常化、减少氧化应激和炎症以及恢复脂质谱来有效改善这些影响。分子对接确定了芦丁、槲皮素和山奈酚等生物活性化合物是谷胱甘肽-S-转移酶、肿瘤坏死因子-α和细胞色素P450的有效抑制剂，其结合亲和力分别为-9.3、-7.2和-8.3千卡/摩尔。这些相互作用支撑了所观察到的抗氧化和抗炎活性。
这些发现表明，鸡冠花和大戟有潜力作为减轻对乙酰氨基酚毒性的天然预防或治疗药物。需要进一步研究以分离其活性化合物，并探索它们与传统治疗方法的协同潜力。本研究架起了传统医学与现代药理学之间的桥梁，为管理药物性肝损伤提供了创新方法。
