苦西瓜果实对扑热息痛诱导的肝毒性的肝保护和抗氧化作用的证据。

Evidences of hepatoprotective and antioxidant effect of Citrullus colocynthis fruits in paracetamol induced hepatotoxicity.

作者信息

Vakiloddin Syed, Fuloria Neeraj, Fuloria Shivkanya, Dhanaraj Sokkalingam Arumugam, Balaji Kaveti, Karupiah Sundram

机构信息

Anuradha College of Pharmacy (University of Amravati), Anuradha Nagar, Chikhli, Dist. Buldana (M.S.), India.

Faculty of Pharmacy, AIMST University, Semeling Campus, Jalan Bedong-Semeling, Bedong, Kedah Darul Aman, Malaysia.

出版信息

Pak J Pharm Sci. 2015 May;28(3):951-7.

PMID:26004728

Abstract

The objective of present study was to explore the hepatoprotective and antioxidant profile of Citrullus colocynthis fruits. Hepatoprotective profile of methanolic extract of Citrullus colocynthis fruits (MECCF) was investigated on rats, which were made hepatotoxic using paracetamol. The antioxidant profile of MECCF was evaluated by conducting Catalase, Super oxide Dismutase, Lipid Peroxidation and Diphenyl Picryl Hydrazyl tests. During hepatoprotective investigation, the Paracetamol treated group II showed significant increase in total bilirubin (TB), serum glutamate oxaloacetate transaminase (SGOT), serum glutamate pyruvate transaminase (SGPT) and alkaline phosphatase (ALP) level. The results so obtained showed that pretreatment of rats with MECCF 300mg/kg p.o. decreases the elevated TB, SGOT, SGPT and ALP serum levels. Also, MECCF inhibitory profile was found comparable with toxicant group (Paracetamol 2g/kg, p.o.). The present study concludes that MECCF fruit possess significant hepatoprotective and antioxidant activity.

摘要

本研究的目的是探究苦西瓜果实的保肝和抗氧化特性。对用扑热息痛造成肝毒性的大鼠研究了苦西瓜果实甲醇提取物（MECCF）的保肝特性。通过进行过氧化氢酶、超氧化物歧化酶、脂质过氧化和二苯基苦味酰基自由基试验评估了MECCF的抗氧化特性。在保肝研究中，扑热息痛处理的第二组总胆红素（TB）、血清谷氨酸草酰乙酸转氨酶（SGOT）、血清谷氨酸丙酮酸转氨酶（SGPT）和碱性磷酸酶（ALP）水平显著升高。所获得的结果表明，用300mg/kg口服MECCF预处理大鼠可降低升高的TB、SGOT、SGPT和ALP血清水平。此外，发现MECCF的抑制特性与中毒组（2g/kg口服扑热息痛）相当。本研究得出结论，MECCF果实具有显著的保肝和抗氧化活性。