Liu Min, Shu Mengmeng, Yan Jiaqing, Liu Xuxu, Wang Ruifeng, Hou Zhiyao, Lin Jun
Department of Periodontology, Stomatological Hospital, Jilin University, Changchun 130021, P. R. China.
Nanoscale. 2021 Jan 14;13(2):1181-1194. doi: 10.1039/d0nr05608a. Epub 2021 Jan 6.
Bone reconstruction is an urgent problem during clinical treatment. In the past few decades, the construction of composite scaffolds has been a hot spot in the research field of bone tissue engineering (BTE). However, the disadvantages of composite materials raise our awareness to explore the potential application of hydroxyapatite (HAp) in bone substitutes due to the closest properties of HAp to natural bone tissue. In our study, we synthesized Eu-doped HAp (HAp:Eu) ultralong nanowires, which can be transformed to hydrophilic net-like scaffolds via a thiol-ene click reaction. The property of luminescence of HAp from Eu is beneficial for identifying the relative position of materials and bone marrow mesenchymal stem cells (BMSCs). HAp:Eu scaffolds with excellent cell biocompatibility could promote the expression of early bone formation markers (ALP and ARS) and enhance the expression of genes and proteins associated with osteogenesis (Runx 2, OCN, and OPN). In the end, the results of the in vivo osteogenesis experiment showed that pure HAp scaffolds presented different effects of bone tissue reconstruction compared with the composite scaffolds with HAp nanorods and polymer materials. The superior osteogenic effect could be observed in net-like pure HAp scaffold groups. Furthermore, the absorption of HAp:Eu scaffolds could be monitored due to the luminescence property of Eu. This strategy based on ultralong HAp nanowires proved to be a new method for the construction of simple reticular scaffolds for potential osteogenic applications.
骨重建是临床治疗中的一个紧迫问题。在过去几十年里，复合支架的构建一直是骨组织工程（BTE）研究领域的热点。然而，由于羟基磷灰石（HAp）与天然骨组织的性质最为接近，复合材料的缺点促使我们探索HAp在骨替代物中的潜在应用。在我们的研究中，我们合成了Eu掺杂的HAp（HAp:Eu）超长纳米线，其可通过硫醇-烯点击反应转化为亲水性网状支架。来自Eu的HAp的发光特性有利于识别材料与骨髓间充质干细胞（BMSCs）的相对位置。具有优异细胞生物相容性的HAp:Eu支架可促进早期骨形成标志物（ALP和ARS）的表达，并增强与成骨相关的基因和蛋白质（Runx 2、OCN和OPN）的表达。最后，体内成骨实验结果表明，与含有HAp纳米棒和聚合物材料的复合支架相比，纯HAp支架呈现出不同的骨组织重建效果。在网状纯HAp支架组中可观察到优越的成骨效果。此外，由于Eu的发光特性，可以监测HAp:Eu支架的吸收情况。这种基于超长HAp纳米线的策略被证明是一种构建简单网状支架用于潜在成骨应用的新方法。
