Hargreaves K, Dubner R, Brown F, Flores C, Joris J
Neurobiology and Anesthesiology Branch, National Institute of Dental Research, National Institutes of Health, Bethesda, MD 20892 U.S.A.
Pain. 1988 Jan;32(1):77-88. doi: 10.1016/0304-3959(88)90026-7.
A method to measure cutaneous hyperalgesia to thermal stimulation in unrestrained animals is described. The testing paradigm uses an automated detection of the behavioral end-point; repeated testing does not contribute to the development of the observed hyperalgesia. Carrageenan-induced inflammation resulted in significantly shorter paw withdrawal latencies as compared to saline-treated paws and these latency changes corresponded to a decreased thermal nociceptive threshold. Both the thermal method and the Randall-Selitto mechanical method detected dose-related hyperalgesia and its blockade by either morphine or indomethacin. However, the thermal method showed greater bioassay sensitivity and allowed for the measurement of other behavioral parameters in addition to the nociceptive threshold.
描述了一种在不受限制的动物中测量对热刺激的皮肤痛觉过敏的方法。测试范式采用行为终点的自动检测；重复测试不会导致所观察到的痛觉过敏的发展。与盐水处理的爪子相比，角叉菜胶诱导的炎症导致爪子缩回潜伏期显著缩短，并且这些潜伏期变化对应于热痛觉阈值的降低。热方法和兰德尔-塞利托机械方法都检测到剂量相关的痛觉过敏及其被吗啡或吲哚美辛阻断。然而，热方法显示出更高的生物测定灵敏度，并且除了痛觉阈值之外还允许测量其他行为参数。
