Research Center for Analytical Sciences, Department of Chemistry, College of Sciences, Northeastern University, Shenyang, 110819, Liaoning, China.
Anal Bioanal Chem. 2021 Feb;413(5):1353-1361. doi: 10.1007/s00216-020-03097-3. Epub 2021 Jan 6.
Copper transporter 1 (CTR1) is a transport protein involved in copper and cisplatin uptake. The visualization of cellular CTR1 migration and its redistribution is highly important in copper/cisplatin exposure/transport. However, to the best of our knowledge, this is a highly challenging task. Herein, a dual-mode imaging strategy for CTR1 is developed by hyphenating confocal laser scanning microscopy (CLSM) and laser ablation inductively coupled plasma mass spectrometry (LA-ICPMS) with a fluorescent/elemental bifunctional tag conjugated with anti-CTR1 antibody. The tag consists of rhodamine B and zirconium metal-organic frameworks (Zr-MOF) for CLSM fluorescence imaging and LA-ICPMS element imaging for a same group of HepG2 cells in a designated visual zone. This dual-mode imaging strategy facilitates visualization of CTR1 migration and meanwhile provides information of CTR1 redistribution in HepG2 cells by uptake of divalent copper or cisplatin. The present dual-mode imaging strategy provides in-depth information for the elucidation of CTR1 involved biological processes. Graphical abstract.
铜转运蛋白 1（CTR1）是一种参与铜和顺铂摄取的转运蛋白。细胞内 CTR1 迁移及其重分布的可视化在铜/顺铂暴露/转运中非常重要。然而，据我们所知，这是一项极具挑战性的任务。在此，通过将共聚焦激光扫描显微镜（CLSM）和激光烧蚀电感耦合等离子体质谱（LA-ICPMS）与荧光/元素双功能标签偶联抗 CTR1 抗体相连接，开发了一种用于 CTR1 的双模成像策略。该标签由罗丹明 B 和锆金属-有机骨架（Zr-MOF）组成，用于 CLSM 荧光成像，以及 LA-ICPMS 元素成像，用于在指定的可视区域对同一组 HepG2 细胞进行成像。这种双模成像策略不仅有助于可视化 CTR1 的迁移，还通过摄取二价铜或顺铂为 HepG2 细胞中 CTR1 的再分布提供了信息。本双模成像策略为阐明 CTR1 参与的生物学过程提供了深入的信息。图摘要。
